Histological and three-dimensional evaluation of osseointegration to nanostructured calcium phosphate-coated implants
Histological and three-dimensional evaluation of osseointegration to nanostructured calcium phosphate-coated implants
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DOI：
10.1016/j.actbio.2011.07.017
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发表时间：
2011-12-01
影响因子：
9.7
通讯作者：
Wennerberg, Ann
中科院分区：
文献类型：
--
作者：
Jimbo, Ryo;Coelho, Paulo G.;Wennerberg, Ann
种植体表面的纳米结构已被证明可以增强骨整合；然而，通常使用的评价技术可能不够敏感，无法充分确定这一过程的影响。本研究旨在通过三维成像和常规组织学相结合的方法观察纳米结构磷酸钙（CaP）包被种植体的骨整合特性。钛植入物采用浸没技术涂覆稳定的CaP纳米颗粒，然后进行热处理。未包被种植体作为对照。经过地形和化学表征后，将植入物插入兔股骨。2周和4周后，取标本进行显微计算机断层扫描和组织形态学评估。扫描电镜评价表明，在纳米尺度上，CaP修饰了植入体表面，获得了具有棒状结构的表面织构。与对照组相比，在种植体手术后4周，cap涂层种植体的骨与种植体接触明显更高。此外，相应的3d图像显示种植体周围有活跃的骨形成。三维定量与二维组织学表现出统计学相关性；此外，3-D量化显示，术后2 - 4周，无涂层对照种植体组骨密度有统计学意义上的下降。三维评价的应用进一步明确了骨内种植体的时间特性和生物反应。(C) 2011材料学报Elsevier Ltd.出版。版权所有。
Nanostructures on implant surfaces have been shown to enhance osseointegration; however, commonly used evaluation techniques are probably not sufficiently sensitive to fully determine the effects of this process. This study aimed to observe the osseointegration properties of nanostructured calcium phosphate (CaP)-coated implants, by using a combination of three-dimensional imaging and conventional histology. Titanium implants were coated with stable CaP nanoparticles using an immersion technique followed by heat treatment. Uncoated implants were used as the control. After topographical and chemical characterizations, implants were inserted into the rabbit femur. After 2 and 4 weeks, the samples were retrieved for micro-computed tomography and histomorphometric evaluation. Scanning electron microscopy evaluation indicated that the implant surface was modified at the nanoscale by CaP to obtain surface textured with rod-shaped structures. Relative to the control, the bone-to-implant contact for the CaP-coated implant was significantly higher at 4 weeks after the implant surgery. Further, corresponding 3-D images showed active bone formation surrounding the implant. 3-D quantification and 2-D histology demonstrated statistical correlation; moreover, 3-D quantification indicated a statistical decrease in bone density in the non-coated control implant group between 2 and 4 weeks after the surgery. The application of 3-D evaluation further clarified the temporal characteristics and biological reaction of implants in bone. (C) 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.