Effect of veratrine and 50 mM K on ileal transport and electrically induced secretion.
Effect of veratrine and 50 mM K on ileal transport and electrically induced secretion.
复制标题
藜芦碱和 50 mM K 对回肠运输和电诱导分泌的影响。
DOI：
10.1152/ajpgi.1981.240.3.g211
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Hubel,KA
中科院分区：
文献类型：
--
作者：
Hubel,KA
为了获得额外的证据表明，体外兔回肠电场刺激（EFS）引起的Cl分泌是由神经或胺前体摄取和脱羧（APUD）细胞介导的，我们确定了是否可以通过去神经，藜芦碱或50 mM K的药物来阻止反应。EFS 1）增加跨壁电位差（PD）和短路电流（Isc），降低总离子电导率（G）; 2）通过减少粘膜-浆膜间的氯离子流而引起氯离子分泌（JClm导致s）单独或与氯化物的血清-粘膜通量增加相结合（JCls导致m），和3）Na吸收减少或通过增加JCls引起的分泌导致单独的m或与JNam减少相关导致s。在10（-5）g/ml时，藜芦碱阻止EFS引起的离子流变化，并降低PD和Isc。然而，它并没有降低对5 mM氨茶碱的Isc反应。50 mmol K降低PD和Isc，增加G. JNam导致s，JNas导致m与浴Na浓度的降低成比例地减小，因此钠的净通量（JNanet）不受影响。JClnet没有变化，尽管JClm导致s和JCls导致m增加。50毫摩尔K阻止了EFS引起的离子通量的变化，并将对氨茶碱的Isc反应降低了一半。这项研究提供了额外的证据，表明EFS的影响是由神经或电兴奋的APUD细胞介导的，但每一个的贡献是不确定的。
To obtain additional evidence that the Cl secretion caused by electrical field stimulation (EFS) in rabbit ileum in vitro is mediated by nerves or amine precursor uptake and decarboxylation (APUD) cells, we determined whether the response could be prevented by agents that depolarize nerves, veratrine or 50 mM K. EFS 1) increased transmural electrical potential difference (PD) and the short-circuit current (Isc) and reduced total ion conductivity (G), 2) caused Cl secretion by reducing the mucosal-to-serosal flow of chloride (JClm leads to s) alone or in combination with an increase in the serosal-to-mucosal flux of chloride (JCls leads to m), and 3) reduced Na absorption or caused secretion by increasing JCls leads to m alone or in association with a reduction in JNam leads to s. At 10(-5) g/ml, veratrine prevented the changes in ionic fluxes caused by EFS and reduced the PD and Isc. It did not reduce the Isc response to 5 mM aminophylline, however. Fifty millimolar K decreased PD and Isc and increased G. JNam leads to s and JNas leads to m diminished in proportion to the reduction in bath Na concentration, so the net flux of sodium (JNanet) was not affected. JClnet did not change, although both JClm leads to s and JCls leads to m increased. Fifty millimolar K prevented the changes in ionic fluxes caused by EFS and reduced the Isc response to aminophylline by half. The study provides additional evidence that the effects of EFS are mediated by nerves or electrically excitable APUD cells, but the contribution of each is uncertain.