Structure of peptide sex pheromone receptor PrgX and PrgX/pheromone complexes and regulation of conjugation in Enterococcus faecalis
Structure of peptide sex pheromone receptor PrgX and PrgX/pheromone complexes and regulation of conjugation in Enterococcus faecalis
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DOI：
10.1073/pnas.0506163102
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发表时间：
2005-12-20
影响因子：
11.1
通讯作者：
Earhart, CA
中科院分区：
文献类型：
--
作者：
Shi, K;Brown, CK;Earhart, CA
许多细菌活动，包括毒力因子的表达、水平遗传转移和抗生素的产生，都是通过使用小分子的细胞间信号传导来控制的。迄今为止，肽介导的细胞-细胞信号传导的分子机制的理解一直受到限制，缺乏有关信号传导组分的分子结构的公开信息。在这里，我们提出了PrgX的分子结构，DNA和肽结合蛋白，调节粪肠球菌质粒pCF 10的接合转移基因的表达，响应细胞间肽信息素信号。PrgX和PrgX/信息素复合物的结构的比较表明，信息素结合不稳定PrgX四聚体，打开共轭阻遏所需的70 bp的pCF 10 DNA环。
Many bacterial activities, including expression of virulence factors, horizontal genetic transfer, and production of antibiotics, are controlled by intercellular signaling using small molecules. To date, understanding of the molecular mechanisms of peptide-mediated cell-cell signaling has been limited by a dearth of published information about the molecular structures of the signaling components. Here, we present the molecular structure of PrgX, a DNA-and peptide-binding protein that regulates expression of the conjugative transfer genes of the Enterococcus faecalis plasmid pCF10 in response to an intercellular peptide pheromone signal. Comparison of the structures of PrgX and the PrgX/pheromone complex suggests that pheromone binding destabilizes PrgX tetramers, opening a 70-bp pCF10 DNA loop required for conjugation repression.