Investigation of thymol effect on learning and memory impairment induced by intrahippocampal injection of amyloid beta peptide in high fat diet- fed rats
Investigation of thymol effect on learning and memory impairment induced by intrahippocampal injection of amyloid beta peptide in high fat diet- fed rats
复制标题
DOI：
10.1007/s11011-017-9960-0
复制
发表时间：
2017-06-01
影响因子：
3.6
通讯作者：
Ebrahim-Habibi, Azadeh
中科院分区：
文献类型：
--
作者：
Asadbegi, Masoumeh;Yaghmaei, Parichehreh;Ebrahim-Habibi, Azadeh
已知肥胖以及高脂肪饮食（HFD）的摄入会增加患阿尔茨海默病（AD）的风险。在本研究中，我们检测了百里香酚（百里香精油中主要的单萜酚）对高脂肪饮食喂养的阿尔茨海默病大鼠模型的保护和治疗作用。40只成年雄性Wistar大鼠随机分为5组（每组8只大鼠）：第1组为对照组，食用普通饮食；第2组食用高脂肪饮食8周，然后通过海马内（IHP）注射磷酸盐缓冲盐水（PBS）；第3组食用高脂肪饮食8周，然后通过海马内注射β - 淀粉样蛋白（Aβ）1 - 42以诱导阿尔茨海默病；第4组食用高脂肪饮食8周，然后注射Aβ1 - 42，并每日用百里香酚（30mg/kg，溶于葵花籽油）治疗4周；第5组食用高脂肪饮食8周，然后注射Aβ1 - 42，之后每日经口灌胃给予葵花籽油，持续4周。生化检测显示，食用高脂肪饮食会导致脂质谱异常和血糖水平升高，而百里香酚治疗可改善这种情况。行为学结果显示，第3组的空间记忆明显受损，但与第3组相比，用百里香酚治疗的组空间记忆更好（p≈0.01）。组织学结果显示，与第1组和第2组相比，第3组海马中Aβ斑块的形成显著增加（p≈0.05），但第4组与第3组相比，Aβ斑块减少（p≈0.01）。总之，百里香酚减轻了Aβ对记忆的影响，可被视为具有神经保护作用。
Obesity and consumption of a high fat diet (HFD) are known to increase the risk of Alzheimer's disease (AD). In the present study, we have examined the protective and therapeutic effects of thymol (main monoterpene phenol found in thyme essential oil) on a HFD-fed rat model of AD. Fourty adult male Wistar rats were randomly assigned to 5 groups:(n = 8 rats/group): group 1, control, consumed an ordinary diet, group 2 consumed a HFD for 8 weeks, then received phosphate-buffered saline (PBS) via intrahippocampal (IHP) injection, group 3 consumed HFD for 8 weeks, then received beta-amyloid (A beta)1-42 via IHP injections to induce AD, group 4 consumed HFD for 8 weeks, then received A beta 1-42, and was treated by thymol (30 mg/kg in sunflower oil) daily for 4 weeks, and group 5 consumed HFD for 8 week, then received A beta 1-42 after what sunflower oil was administered by oral gavage daily for 4 weeks. Biochemical tests showed an impaired lipid profile and higher glucose levels upon consumption of HFD, which was ameliorated by thymol treatment. In behavioral results, spatial memory in group 3 was significantly impaired, but groups treated with thymol showed better spatial memory compared to group 3 (p ae 0.01). In histological results, formation of A beta plaque in hippocampus of group 3 increased significantly compared to group 1 and group 2 (p ae 0.05), but group 4 showed decreased A beta plaques compared to group 3 (p ae 0.01). In conclusion, thymol decreased the effects of A beta on memory and could be considered as neuroprotective.