Assessment of biological characteristics of mesenchymal stem cells labeled with superparamagnetic iron oxide particles in vitro.
Assessment of biological characteristics of mesenchymal stem cells labeled with superparamagnetic iron oxide particles in vitro.
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DOI：
10.3892/mmr.2011.637
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发表时间：
2011-10
影响因子：
3.4
通讯作者：
Junhui Sun;Yue-Lin Zhang;Su-Ping Qian;Xiaobo Yu;Haiyang Xie;Lin Zhou;Shu-sen Zheng
中科院分区：
文献类型：
--
作者：
Junhui Sun;Yue-Lin Zhang;Su-Ping Qian;Xiaobo Yu;Haiyang Xie;Lin Zhou;Shu-sen Zheng
间充质干细胞(MSC)移植为人类疾病的治疗提供了新的策略。磁共振成像(MRI)能够在体内追踪标记了超顺磁性氧化铁(SPIO)的移植干细胞。然而，SPIO对标记的MSCs的作用在细胞水平上仍不清楚。本研究对自制SPIO颗粒标记的大鼠骨髓间充质干细胞的生物学特性进行了研究。从5只成年SD大鼠的骨髓中分离出MSCs，用自制的SPIO颗粒进行铁终末浓度为20µg/ml的标记，用普鲁士蓝染色和透射电子显微镜观察。用原子吸收光谱法测定细胞内的铁含量。比较标记与未标记间充质干细胞的细胞活力、增殖、膜抗原及多向分化能力。普鲁士蓝染色显示大鼠骨髓间充质干细胞得到了有效的标记，标记率接近100%。透射电子显微镜证实，SPIO颗粒位于MSCs的内体囊泡中。标记和未标记的MSCs在细胞活力、增殖、膜抗原和多向分化能力等方面均无显著差异(P>；0.05)。综上所述，自制的SPIO颗粒能够在不影响MSCs主要生物学特性的情况下，有效地标记MSCs。
Mesenchymal stem cell (MSC) transplantation provides a novel strategy for the treatment of human disease. MR imaging (MRI) is able to track transplanted stem cells labeled with superparamagnetic iron oxide (SPIO) in vivo. However, the effect of SPIO upon labeled MSCs remains unclear on a cellular level. In this study, the biological characteristics of rat MSCs labeled with home-synthesized SPIO particles were evaluated. The MSCs were isolated from the bone marrow of 5 adult Sprague-Dawley rats and labeled with home-synthesized SPIO particles at a final iron concentration of 20 µg/ml. Labeled MSCs were confirmed with Prussian blue staining and transmission electron microscopy. The quantity of iron per cell was determined by atomic absorption spectrometry. Cell viability, proliferation, membranous antigen and multiple differentiation ability were compared between labeled and unlabeled MSCs. The rat MSCs were effectively labeled and the labeling efficiency was approximately 100%, as revealed by Prussian blue staining. The SPIO particles located in the endosomal vesicles of the MSCs were confirmed by transmission electron microscopy. No significant differences were observed in cell viability, proliferation, membranous antigen and multiple differentiation ability between the labeled and unlabeled MSCs (P>0.05). In conclusion, MSCs are able to be effectively labeled by home-synthesized SPIO particles without influencing their main biological characteristics.