Adult zebrafish Langerhans cells arise from hematopoietic stem/progenitor cells.
Adult zebrafish Langerhans cells arise from hematopoietic stem/progenitor cells.
复制标题
作者：
He S;Chen J;Jiang Y;Wu Y;Zhu L;Jin W;Zhao C;Yu T;Wang T;Wu S;Lin X;Qu JY;Wen Z;Zhang W;Xu J
朗格汉斯细胞（LC）是皮肤表皮驻留的巨噬细胞，其起源仍不清楚。目前LC的谱系追踪主要依赖于启动子-Cre-LoxP系统，这经常导致不同启动子的相互矛盾的结论。因此，有必要用改进的追踪方法重新调查。在这里，使用激光介导的时空分辨细胞标记方法，我们证明了大多数成年LC起源于背主动脉（VDA）的腹侧壁，相当于小鼠主动脉，性腺和中肾（AGM），其中生成造血干细胞（HSC）和非HSC祖细胞。进一步的精细命运定位分析显示，成年斑马鱼中LC的出现与HSC的发育相关，但与T细胞祖细胞无关。最后，我们发现成年斑马鱼的脑、肝、心脏和肠道中的组织驻留巨噬细胞的出现也与HSC相关。因此，我们的研究结果对LC的EMP起源理论提出了挑战。
The origin of Langerhans cells (LCs), which are skin epidermis-resident macrophages, remains unclear. Current lineage tracing of LCs largely relies on the promoter-Cre-LoxP system, which often gives rise to contradictory conclusions with different promoters. Thus, reinvestigation with an improved tracing method is necessary. Here, using a laser-mediated temporal-spatial resolved cell labeling method, we demonstrated that most adult LCs originated from the ventral wall of the dorsal aorta (VDA), an equivalent to the mouse aorta, gonads, and mesonephros (AGM), where both hematopoietic stem cells (HSCs) and non-HSC progenitors are generated. Further fine-fate mapping analysis revealed that the appearance of LCs in adult zebrafish was correlated with the development of HSCs, but not T cell progenitors. Finally, we showed that the appearance of tissue-resident macrophages in the brain, liver, heart, and gut of adult zebrafish was also correlated with HSCs. Thus, the results of our study challenged the EMP-origin theory for LCs.