Gene expression profiles of chemosensory genes of termite soldier and worker antennae
Gene expression profiles of chemosensory genes of termite soldier and worker antennae
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DOI：
10.1111/imb.12841
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发表时间：
2023-04-05
影响因子：
2.6
通讯作者：
Hojo，Masaru K.
中科院分区：
文献类型：
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作者：
Suzuki，Ryohei H.;Hanada，Takumi;Hojo，Masaru K.
白蚁有一个复杂的社会系统，包括殖民地成员之间的合作和分工。虽然这种社会系统是由殖民地产生的化学信号调节的，但其他成员如何感知这些信号仍不清楚。众所周知，信号转导是由触角中的一些结合蛋白接收气味分子触发的，之后，信号被传递到化学感受器。然而，在白蚁中参与信号转导的化学感受基因的作用的信息不足。在这里，我们确定了白蚁Reticulitermes speratus中参与化学感受器接收的基因，并对工蚁和兵蚁触角进行了全基因组比较转录组分析。首先，我们从基因组数据中鉴定了31种气味结合蛋白（OBP）和3种化学感受蛋白A（CheA）。此后，我们进行了RNA测序，以比较OBP，CheAs和先前确定的化学感受器基因在工蜂和士兵触角之间的表达水平。没有受体基因的表达在种姓之间的显着差异。然而，三种非受体气味检测/结合蛋白（OBP，CheA和感觉神经元膜蛋白）的表达水平在种姓之间存在显著差异。使用触角和其他头部部分的真实的实时qPCR（RT-qPCR）分析证实，这些基因在士兵触角中高度表达。最后，独立的RT-qPCR分析表明，这些基因的表达模式在来自不同社会背景的士兵中发生了改变。目前的研究结果表明，一些非受体的基因表达水平受到白蚁群体成员之间的种姓和行为相互作用的影响。
Termites have an elaborate social system that involves cooperation and division of labour among colony members. Although this social system is regulated by chemical signals produced in the colony, it remains unclear how these signals are perceived by other members. Signal transduction is well known to be triggered by the reception of odorant molecules by some binding proteins in the antennae, after which, a signal is transmitted to chemosensory receptors. However, there is insufficient information on the role of chemosensory genes involved in signal transduction in termites. Here, we identified the genes involved in chemosensory reception in the termiteReticulitermes speratusand performed a genome‐wide comparative transcriptome analysis of worker and soldier antennae. First, we identified 31 odorant‐binding proteins (OBPs), and three chemosensory protein A (CheA) from the genome data. Thereafter, we performed RNA sequencing to compare the expression levels ofOBPs,CheAs, and previously identified chemosensory receptor genes between worker and soldier antennae. There were no receptor genes with significant differences in expression between castes. However, the expression levels of three non‐receptor odorant‐detection/binding proteins (OBP,CheA,andSensory neuron membrane protein) were significantly different between castes. Real‐time qPCR (RT‐qPCR) analysis using antennae and other head parts confirmed that these genes were highly expressed in soldier antennae. Finally, independent RT‐qPCR analysis showed that the expression patterns of these genes were altered in soldiers from different social contexts. Present results suggest that gene expression levels of some non‐receptors are affected by both castes and behavioural interactions among colony members in termites.