Type I repressors of P element mobility.
Type I repressors of P element mobility.
复制标题
P 元件移动性的 I 型阻遏物。
DOI：
10.1093/genetics/135.1.81
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发表时间：
1993
期刊：
影响因子：
3.3
通讯作者：
Engels,WR
中科院分区：
文献类型：
--
作者：
Gloor,GB;Preston,CR;Johnson-Schlitz,DM;Nassif,NA;Phillis,RW;Benz,WK;Robertson,HM;Engels,WR
我们在这里描述了一个家庭的P元素，我们称之为I型阻遏物。这些元件通过它们的阻遏物功能和它们在经典P序列的前三分之二内缺乏任何缺失来鉴定。从P菌株中分离出属于这类阻遏物的元件，并在体外制备。我们发现，I型阻遏元件可以强烈抑制细胞型依赖的等位基因和P元素的体细胞和种系组织的流动性。这些效应非常依赖于基因组位置。此外，我们观察到，一个元素的抑制能力，在一个测定与它的抑制能力，在其他两个测定中的任何一个呈正相关。I型阻遏元件家族包括自主的P元件和缺乏P元件的外显子3的元件。精细结构缺失作图显示功能性I型元件的最小3'边界位于核苷酸位置1950和1956之间。在12个元件中，没有一个元件具有延伸到外显子2的更极端的缺失。我们的结论是，I型阻遏物形成了一个结构上不同的组，不包括更广泛地删除阻遏物元素，如KP元素前面所述。
We describe here a family of P elements that we refer to as type I repressors. These elements are identified by their repressor functions and their lack of any deletion within the first two-thirds of the canonical P sequence. Elements belonging to this repressor class were isolated from P strains and were made in vitro. We found that type I repressor elements could strongly repress both a cytotype-dependent allele and P element mobility in somatic and germline tissues. These effects were very dependent on genomic position. Moreover, we observed that an element's ability to repress in one assay positively correlated with its ability to repress in either of the other two assays. The type I family of repressor elements includes both autonomous P elements and those lacking exon 3 of the P element. Fine structure deletion mapping showed that the minimal 3' boundary of a functional type I element lies between nucleotide position 1950 and 1956. None of 12 elements examined with more extreme deletions extending into exon 2 made repressor. We conclude that the type I repressors form a structurally distinct group that does not include more extensively deleted repressor elements such as the KP element described previously.