Rapid and sensitive detection of human African trypanosomiasis by loop-mediated isothermal amplification combined with a lateral-flow dipstick
Rapid and sensitive detection of human African trypanosomiasis by loop-mediated isothermal amplification combined with a lateral-flow dipstick
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DOI：
10.1016/j.diagmicrobio.2010.08.026
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发表时间：
2011-02-01
影响因子：
2.9
通讯作者：
Njiru, Zablon Kithinji
中科院分区：
文献类型：
--
作者：
Njiru, Zablon Kithinji
采用环介导的等温扩增侧流试纸（LAMP-LED）格式对临床样品中人类感染性锥虫DNA的检测进行了评估。LAMP-LFD的分析灵敏度相当于0.01 tryps/mL，与凝胶电泳和SYBR (R) Green I染料的分析灵敏度相同。LAMP-LFD以煮沸的人类生物样品制备的上清为模板，对SYBR (R) Green 1具有较好的特异性。这些结果表明，当使用部分处理过的模板时，使用非特异性DNA插入物可能会产生假阳性。LAMP-LFD格式简单、快速，在昏睡病的诊断中有潜在的应用前景。(C) 2011爱思唯尔公司版权所有。
A combined loop-mediated isothermal amplification lateral flow dipstick (LAMP-LED) format was evaluated in the detection of human infective trypanosome DNA from clinical samples. The LAMP-LFD showed analytical sensitivity equivalent to 0.01 tryps/mL, levels that were identical to using gel electrophoresis and SYBR (R) Green I dye. The LAMP-LFD showed superior specificity to SYBR (R) Green 1 when supernatant prepared from boiled human biological samples was used as template. These results indicate that the use of nonspecific DNA intercalators may produce false positives when partially processed templates are used. The LAMP-LFD format presented here is simple, rapid, and has future potential use in diagnosis of sleeping sickness. (C) 2011 Elsevier Inc. All rights reserved.