The superoxide dismutase genes might be required for appropriate development of the ovule after fertilization in Xanthoceras sorbifolium
The superoxide dismutase genes might be required for appropriate development of the ovule after fertilization in Xanthoceras sorbifolium
复制标题
文冠果受精后胚珠的正常发育可能需要超氧化物歧化酶基因
DOI：
10.1007/s00299-018-2263-z
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发表时间：
2018-01
影响因子：
6.2
通讯作者：
Cai Qing
中科院分区：
文献类型：
--
作者：
Zhou Qingyuan;Cai Qing
超氧化物歧化酶（SOD）基因在文冠果受精胚珠发育过程中沿着差异表达，XsMSD基因沉默导致受精胚珠发育受阻。。摘要一个非常小的百分比成熟的水果（约。5%）是相对于文冠果中两性花的数量产生的，因为种子和果实在授粉后的早期发育阶段就败育了。植物体内的活性氧（ROS）参与了广泛的生物学过程，如细胞程序性死亡和衰老。超氧化物歧化酶（SOD）活性可能是调节受精胚珠中ROS稳态所必需的。山梨叶本研究鉴定了5个SOD基因和1个SOD铜伴侣基因。它们的转录本沿着受精胚珠发育的不同阶段差异表达。这些基因在授粉后3天（DAP）胚珠中表达最多，此时游离核胚乳和核组织迅速发育。XsCSD 1、XsFSD 1和XsMSD分别含有7、8和5个内含子。对XsFSD 1和XsMSD的5 '侧翼区的分析揭示了许多顺式作用调控元件。基于病毒诱导的基因沉默（VIGS）的XsMSD基因功能的评估表明，该基因与受精胚珠和果实的早期发育密切相关。本研究表明，SOD基因可能与受精后胚珠发育的命运（败育或存活）密切相关。山梨叶
Key message Superoxide dismutase genes were expressed differentially along with developmental stages of fertilized ovules in Xanthoceras sorbifolium, and the XsMSD gene silencing resulted in the arrest of fertilized ovule development. .Abstract A very small percentage of mature fruits (ca. 5%) are produced relative to the number of bisexual flowers in Xanthoceras sorbifolium because seeds and fruits are aborted at early stages of development after pollination. Reactive oxygen species (ROS) in plants are implicated in an extensive range of biological processes, such as programmed cell death and senescence. Superoxide dismutase (SOD) activity might be required to regulate ROS homeostasis in the fertilized ovules of X. sorbifolium. The present study identified five SOD genes and one SOD copper chaperone gene in the tree. Their transcripts were differentially expressed along different stages of fertilized ovule development. These genes showed maximum expression in the ovules at 3 days after pollination (DAP), a time point in which free nuclear endosperm and nucleus tissues rapidly develop. The XsCSD1, XsFSD1 and XsMSD contained seven, eight, and five introns, respectively. Analysis of the 5’-flanking region of XsFSD1 and XsMSD revealed many cis-acting regulatory elements. Evaluation of XsMSD gene function based on virus-induced gene silencing (VIGS) indicated that the gene was closely related to early development of the fertilized ovules and fruits. This study suggested that SOD genes might be closely associated with the fate of ovule development (aborted or viable) after fertilization in X. sorbifolium..
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DOI：
--
发表时间：
1999-10
期刊：
Botanical Bulletin of Academia Sinica
影响因子：
--
作者：
S. Pan;Guan-Bor Hwang;Hung-Chi Liu
通讯作者：
S. Pan;Guan-Bor Hwang;Hung-Chi Liu
DOI：
10.1073/pnas.87.24.9903
发表时间：
1990-12
影响因子：
11.1
作者：
W. V. Camp;C. Bowler;R. Villarroel;W. Tsang;M. MONTAGUt;Dirk INZt
通讯作者：
W. V. Camp;C. Bowler;R. Villarroel;W. Tsang;M. MONTAGUt;Dirk INZt
影响因子：
7.4
作者：
MCCORD, JM;FRIDOVICH, I
通讯作者：
FRIDOVICH, I
DOI：
10.1016/0167-4781(88)90025-5
发表时间：
1988-11
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
J. A. White;J. G. Scandalios
通讯作者：
J. A. White;J. G. Scandalios
影响因子：
11.4
作者：
Li, Wen;Qi, Lin;Huang, Shangzhi
通讯作者：
Huang, Shangzhi