The endogenous redox rhythm is controlled by a central circadian oscillator in cyanobacterium Synechococcus elongatus PCC7942
The endogenous redox rhythm is controlled by a central circadian oscillator in cyanobacterium Synechococcus elongatus PCC7942
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内源性氧化还原节律由蓝细菌细长聚球藻 PCC7942 中的中央昼夜节律振荡器控制
DOI：
10.1007/s11120-019-00667-0
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发表时间：
2019
影响因子：
3.7
通讯作者：
Shuji Nakanishi
中科院分区：
文献类型：
--
作者：
Kenya Tanaka;Masahito Ishikawa;Masahiro Kaneko;Kazuhide Kamiya;Souichiro Kato;Shuji Nakanishi
光合作用生物的细胞内氧化还原和生物钟是全球影响各种生物功能的两个主要调节因子。这两个全球控制系统都已演变为适应规则或不规则变化的光环境的系统。在这里，我们报道了这两个全球调控因子在蓝藻PCC7942中相互作用，PCC7942是一个模式光合作用生物，其时钟分子机制是众所周知的。使用跨膜电子介体的电化学测试表明，细胞内的氧化还原OFS。PCC7942细胞在恒定光照条件下表现出昼夜节律。当Clock基因的转录/翻译去功能化时，氧化还原节律消失，这表明转录/翻译由核心KaiABC振荡器控制，产生昼夜氧化还原节律。重要的是，在恒定的光条件下，氧化还原节奏的幅度足够大，足以影响KaiABC振荡器。研究结果表明，在恒定光照条件下，细胞内氧化还原状态被生物钟系统主动控制为24小时周期内的变化。
The intracellular redox and the circadian clock in photosynthetic organisms are two major regulators globally affecting various biological functions. Both of the global control systems have evolved as systems to adapt to regularly or irregularly changing light environments. Here, we report that the two global regulators mutually interact in cyanobacteriumSynechococcus elongatusPCC7942, a model photosynthetic organism whose clock molecular mechanism is well known. Electrochemical assay using a transmembrane electron mediator revealed that intracellular redox ofS. elongatusPCC7942 cell exhibited circadian rhythms under constant light conditions. The redox rhythm disappeared when transcription/translation of clock genes is defunctionalized, indicating that the transcription/translation controlled by a core KaiABC oscillator generates the circadian redox rhythm. Importantly, the amplitude of the redox rhythm at a constant light condition was large enough to affect the KaiABC oscillator. The findings indicated that the intracellular redox state is actively controlled to change in a 24-h cycle under constant light conditions by the circadian clock system.