Effects of temperature and wetness duration on conidial infection, latent period and asexual sporulation of Pyrenopeziza brassicae on leaves of oilseed rape
Effects of temperature and wetness duration on conidial infection, latent period and asexual sporulation of Pyrenopeziza brassicae on leaves of oilseed rape
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温度和湿润时间对油菜叶片分生孢子侵染、潜伏期和无性产孢的影响
DOI：
10.1046/j.1365-3059.2000.00474.x
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发表时间：
2000
期刊：
影响因子：
2.7
通讯作者：
M. Jeger
中科院分区：
文献类型：
--
作者：
T. Gilles;B. Fitt;R. Kennedy;S. Welham;M. Jeger
在受控环境中进行实验，以确定温度和叶湿润持续时间对油菜叶被轻叶斑病病原体 Pyrenopeziza bassicae 的分生孢子侵染的影响。 cv.的叶子上发育可见的孢子脓疱。 Bristol 在 4 至 20 摄氏度的温度下接种 P. 芸苔分生孢子，但不在 24 摄氏度下接种；当接种后叶片湿润持续时间长于或等于约12-20℃下6小时、8℃下10小时、6℃下16小时或4℃下24小时时，孢子脓疱形成。当接种后叶片湿润持续时间分别大于3小时或24小时时，摩羯座或眼镜蛇会在8摄氏度和16摄氏度时出现轻微叶斑病症状。甘蓝假单胞菌在 cv 上的潜伏期（从接种到第一个孢子脓疱的时间段）。布里斯托尔在 16 摄氏度时，叶片湿润持续时间为 24 小时，平均约为 10 天；当温度升高至 20 摄氏度时，叶片湿润持续时间增加至约 12 天；当温度降至 4 摄氏度时，叶片湿润持续时间增加至 26 天。在 8 摄氏度时，叶片湿润持续时间从 10 小时增加至 72 小时，潜伏期从约 25 天减少至 16 天；在 6 摄氏度下，叶片湿润持续时间从 16 小时增加到 72 小时，潜伏期从大约 23 天减少到 17 天。 12-16℃时产生的分生孢子数量最多，并随着温度降低至8℃或升高至20℃而减少。在8℃至20℃的温度下，叶湿润时间从6小时增加至24小时增加了分生孢子的产量。在不同温度下，叶子上产生的分生孢子数量与被“病斑”（均为log(10)变换）覆盖的叶面积比例之间存在线性关系。
Experiments in controlled environments were carried out to determine the effects of temperature and leaf wetness duration on infection of oilseed rape leaves by conidia of the light leaf spot pathogen, Pyrenopeziza brassicae. Visible spore pustules developed on leaves of cv. Bristol inoculated with P. brassicae conidia at temperatures from 4 to 20 degrees C, but not at 24 degrees C; spore pustules developed when the leaf wetness duration after inoculation was longer than or equal to approximately 6 h at 12-20 degrees C, 10 h at 8 degrees C, 16 h at 6 degrees C or 24 h at 4 degrees C. On leaves of cvs. Capricorn or Cobra, light leaf spot symptoms developed at 8 and 16 degrees C when the leaf wetness duration after inoculation was greater than 3 or 24 h, respectively. The latent period (the time period from inoculation to first spore pustules) of P. brassicae on cv. Bristol was, on average, approximately 10 days at 16 degrees C when leaf wetness duration was 24 h, and increased to approximately 12 days as temperature increased to 20 degrees C and to 26 days as temperature decreased to 4 degrees C. At 8 degrees C, an increase in leaf wetness duration from 10 to 72 h decreased the latent period from approximately 25 to 16 days; at 6 degrees C, an increase in leaf wetness duration from 16 to 72 h decreased the latent period from approximately 23 to 17 days. The numbers of conidia produced were greatest at 12-16 degrees C, and decreased as temperature decreased to 8 degrees C or increased to 20 degrees C. At temperatures from 8 to 20 degrees C, an increase in leaf wetness duration from 6 to 24 h increased the production of conidia. There were linear relationships between the number of conidia produced on a leaf and the proportion of the leaf area covered by 'lesions' (both log(10)-transformed) at different temperatures.