Expression of MicroRNA machinery proteins in different types of chronic rhinosinusitis
Expression of MicroRNA machinery proteins in different types of chronic rhinosinusitis
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不同类型慢性鼻-鼻窦炎中microRNA机械蛋白的表达
DOI：
10.1002/lary.23517
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发表时间：
2012-12-01
期刊：
影响因子：
2.6
通讯作者：
Liu, Zheng
中科院分区：
文献类型：
--
作者：
Zhang, Ya-Na;Cao, Ping-Ping;Liu, Zheng
目标/假设：最近在慢性鼻窦炎 (CRS) 中发现了 microRNA (miRNA) 的失调，其生物发生和功能受到 miRNA 机械蛋白的调节。这些蛋白质在炎症性气道疾病中的表达尚不清楚。本研究的目的是研究 CRS 中 miRNA 机械成分的表达。研究设计：病例对照实验研究。方法：对照鼻活检、无鼻息肉的 CRS (CRSsNP)、嗜酸性粒细胞和非嗜酸性粒细胞 CRS 中 miRNA 机械成分的 mRNA 表达水平，包括 Drosha、Dicer、干扰素诱导蛋白激酶 (PACT) 的蛋白激活剂、人类免疫缺陷病毒反式激活反应 RNA 结合蛋白、脆性 X 智力迟钝蛋白和 argonaute 2/真核翻译起始因子 2C, 2患有鼻息肉（CRSwNP）的受试者通过定量逆转录聚合酶链反应进行测定。采用免疫组织化学染色检查PACT的蛋白表达和PACT的细胞来源。结果：在测试的成分中，仅发现 CRS 中 PACT mRNA 表达发生改变，与对照相比，CRSwNP 中 PACT mRNA 表达水平上调。与对照和 CRSsNP 相比，CRSwNP 中 PACT 蛋白表达也显着上调，且嗜酸性 CRSwNP 中进一步增加。 PACT主要表达于CD138+浆细胞。在嗜酸性 CRSwNP 中检测到的 PACT 阳性浆细胞占总浆细胞的百分比高于非嗜酸性 CRSwNP。 PACT 蛋白表达与疾病严重程度和嗜酸性粒细胞浸润相关。结论：PACT可能与CRSwNP的浆细胞功能和嗜酸性炎症有关。然而，需要进一步的实验来阐明 PACT 的功能。
Objectives/Hypothesis: Dysregulation of microRNAs (miRNAs) has recently been shown in chronic rhinosinusitis (CRS), the biogenesis and function of which are modulated by miRNA machinery proteins. The expression of these proteins in inflammatory airway diseases is unclear. The aim of this study was to investigate the expression of miRNA machinery components in CRS. Study Design: Casecontrol experimental study. Methods: The mRNA expression levels of miRNA machinery components including Drosha, Dicer, protein activator of the interferon-induced protein kinase (PACT), human immunodeficiency virus transactivating response RNA-binding protein, fragile X mental retardation protein, and argonaute 2/eukaryotic translation initiation factor 2C, 2 in nasal biopsies from control, CRS without nasal polyps (CRSsNP), eosinophilic, and noneosinophilic CRS with nasal polyps (CRSwNP) subjects were determined by quantitative reverse transcription polymerase chain reaction. Immunohistochemical staining was employed to examine the protein expression of PACT and the cellular source of PACT. Results: Among the tested components, only PACT mRNA expression was found to be altered in CRS, the levels of which were upregulated in CRSwNP as compared with control. In comparison with control and CRSsNP, PACT protein expression was also significantly upregulated in CRSwNP, with a further increase in eosinophilic CRSwNP. PACT was mainly expressed in CD138+ plasma cells. A higher percentage of PACT-positive plasma cells in total plasma cells was detected in eosinophilic CRSwNP than in noneosinophilic CRSwNP. PACT protein expression correlated with disease severity and eosinophil infiltration. Conclusions: PACT may be associated with the plasma cell function and eosinophilic inflammation in CRSwNP. However, further experimentation is needed to clarify the functions of PACT.