Estrogen receptor-β expression in relation to the expression of luteinizing hormone receptor and cytochrome P450 enzymes in rat ovarian follicles
Estrogen receptor-β expression in relation to the expression of luteinizing hormone receptor and cytochrome P450 enzymes in rat ovarian follicles
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DOI：
10.1095/biolreprod63.6.1747
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发表时间：
2000-12-01
影响因子：
3.6
通讯作者：
Sundaram, K
中科院分区：
文献类型：
--
作者：
Bao, B;Kumar, N;Sundaram, K
研究了动情前期大鼠卵泡颗粒细胞和卵泡膜细胞中雌激素受体（ERP）mRNA表达与LH受体（LHr）和细胞色素P450酶mRNAs表达的关系。从15只成年大鼠采集的30个卵巢中，24个用于原位杂交，其余用于逆转录-聚合酶链反应。ERP，LHr，细胞色素P450侧链裂解酶（P450（SCC）），17 α-羟化酶（P450（c17）），芳香化酶（P450（arom））和类固醇生成急性调节蛋白（星星）的信使RNA通过原位杂交定位在卵巢的横截面中，并通过计算机图像分析系统在颗粒层和卵泡膜细胞层中定量。卵巢卵泡分为健康卵泡和闭锁卵泡。将健康卵泡分为四个大小组：非常小（40-100 μ m）、小（101-275 μ m）、中（276-450 μ m）和大（451-850 μ m）。闭锁卵泡分为中等（276-450 μ m）或大卵泡（451-850 μ m）。ERPmRNA的低水平表达首先在非常小的健康卵泡的颗粒细胞中检测到，并且表达逐渐增加到中等大小的卵泡。ERPmRNA在中等大小卵泡中表达最高（P < 0.01），大卵泡中表达最低（P < 0.01）。LHr、P450（SCC）和P450（arom）的信使RNA首先在中等大小的健康卵泡的颗粒细胞中检测到，而LHr、P450、P450、和星星的mRNA首先在与非常小的卵泡相关的卵泡膜细胞中检测到。LHr、P450（SCC）、P450（c17）、P450（arom）和星星的最高表达见于大健康卵泡的颗粒细胞和/或卵泡膜细胞。在闭锁卵泡中，颗粒细胞和卵泡膜细胞的基因表达水平相对较低。总之，在卵泡发育过程中，颗粒细胞中观察到ERP mRNA的阶段特异性表达。ERP的表达增加，并伴随启动LHr，P450（SCC），P450（arom）的表达在中等卵泡的颗粒细胞可能意味着在卵泡发育中的雌激素的作用。此外，颗粒细胞中ERP mRNA表达与卵泡膜细胞中LHr、P450（SCC）、P450（C17）和星星mRNA表达之间的强相关性表明雌激素在类固醇生成中可能发挥作用。
Changes in mRNA expression for estrogen receptor (ERP) in relation to mRNAs for LH receptor (LHr) and cytochrome P450 enzymes were examined in granulosa and theca cells from proestrous rat ovarian follicles. Of the 30 ovaries harvested from 15 adult rats, 24 were processed for in situ hybridization, and the remaining were used for reverse transcription-polymerase chain reaction. Messenger RNAs for ERP, LHr, cytochrome P450 side-chain cleavage enzyme (P450(scc)), 17 alpha -hydroxylase (P450(c17)), aromatase (P450(arom)), and steroidogenic acute regulatory protein (StAR) were localized in cross sections of ovaries by in situ hybridization and quantified in granulosa and theca cell layers by a computer-image analyzing system. Ovarian follicles were classified as healthy or atretic. Healthy follicles were divided into four size groups: very small (40-100 mum), small (101-275 mum), medium (276-450 mum), and large (451-850 pm). Atretic follicles were divided into medium (276-450 pm) or large follicles (451-850 mum). A low level of ERP mRNA expression was first detected in granulosa cells of very small healthy follicles, and the expression increased progressively up to medium-sized follicles. The expression of ERP mRNA was highest (P < 0.01) in medium-sized follicles that was followed by a decrease (P < 0.01) in large follicles. Messenger RNAs for LHr, P450(scc), and P450(arom) were first detected in granulosa cells of medium-sized healthy follicles, while mRNAs for LHr, P450,,,, P450,,,, and StAR were first detected in theca cells associated with very small follicles. The highest expression of LHr, P450(scc), P450(c17), P450(arom), and StAR was seen in granulosa and/or theca cells of large healthy follicles. In atretic follicles, level of gene expression was relatively low in both granulosa and theca cells. In conclusion, stage-specific expression of ERP mRNA was observed in granulosa cells during follicular development. The increased expression of ERP and a concomitant initiation of LHr, P450(scc), and P450(arom) expression in granulosa cells of medium follicles may signify a role for estrogen in follicular development. Also, a strong correlation between ERP mRNA expression in granulosa cells, and the expression of mRNAs for LHr, P450(scc), P450(c17), and StAR in theca cells associated with growing follicles suggests a possible role for estrogen in steroidogenesis.