Chromosomal variation and the establishment of somatic cell lines in vitro
Chromosomal variation and the establishment of somatic cell lines in vitro
复制标题
染色体变异与体外体细胞系的建立
DOI：
10.1038/253361a0
复制
发表时间：
1975
期刊：
影响因子：
64.8
通讯作者：
T. J. Hawkins
中科院分区：
文献类型：
--
作者：
M. Terzi;T. J. Hawkins
当一些物种的体细胞在体外移植时，它们可能要么完全无法生长，要么可能继续分裂到有限的世代，分裂的速度逐渐降低，培养效率下降。他们最终会死亡，除非从这场“危机”中，出现一个新的人口与改变的财产(见参考文献。1)。这个新的种群，即已建立的生产线，通常能够无限期地存在，其增长速度和电镀效率逐渐增加，直到它们稳定在每个单独生产线特有的水平。
WHEN somatic cells from a number of species are explanted in vitro, they may either completely fail to grow, or they may continue to divide for a limited number of generations, at a progressively lower rate and with decreasing plating efficiency. They will eventually die unless, from this “crisis”, a new population emerges with altered properties (see for example ref. 1). This new population, the established line, is usually capable of existing indefinitely and its growth rate and plating efficiency progressively increase until they stabilise at a level characteristic of each individual line.