Virulent Burkholderia pseudomallei is more efficient than avirulent Burkholderia thailandensis in invasion of and adherence to cultured human epithelial cells
Virulent Burkholderia pseudomallei is more efficient than avirulent Burkholderia thailandensis in invasion of and adherence to cultured human epithelial cells
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DOI：
10.1016/j.micpath.2004.01.001
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发表时间：
2004-05-01
影响因子：
3.8
通讯作者：
Sirisinha, S
中科院分区：
文献类型：
--
作者：
Kespichayawattana, W;Intachote, P;Sirisinha, S
类鼻疽伯克霍尔德杆菌是类鼻疽的病原体，是一种兼性细胞内革兰氏阴性杆菌，与其无毒对应物泰国伯克霍尔德杆菌密切相关。然而，类鼻疽伯克氏菌的致病机制和毒力因子仍不清楚。在本研究中，我们比较了类鼻疽伯克氏菌和泰国鼻疽伯克氏菌在人呼吸道上皮细胞 A549 中的侵袭性、粘附性和复制能力。共培养 4 小时后，使用抗生素保护测定法测定侵袭情况。通过将细胞与荧光素标记的细菌共培养1小时来证明粘附，并通过流式细胞术分析阳性细胞的数量。这项体外研究的结果表明，与无毒对应物相比，类鼻疽杆菌在侵袭、粘附和诱导细胞损伤（以斑块形成为代表）方面显着更有效（P < 0.01）。 (C) 2004 Elsevier Ltd. 保留所有权利。
Burkholderia pseudomallei, a causative agent of melioidosis, is a facultative intracellular gram-negative bacillus that is closely related to its avirulent counterpart, Burkholderia thailandensis. However, pathogenic mechanisms and virulence factors of B. pseudomallei remain elusive. In the present study, we compared the invasiveness, adherence, and replication of B. pseudomallei and B. thailandensis in human respiratory epithelial cells A549. Invasion was determined after 4 h of coculturing using antibiotic protection assay. Adherence was demonstrated by coculturing the cells with fluorescein-labeled bacteria for I h and the number of positive cells was analyzed by flow cytometry. The results obtained with this in vitro study demonstrated that compared with its avirulent counterpart, B. pseudomallei is significantly more efficient (P < 0.01) in invasion, adherence and inducing cellular damage, as represented by plaque formation. (C) 2004 Elsevier Ltd. All rights reserved.