Augmenter of liver regeneration promotes hepatic regeneration depending on the integrity of Kupffer cell in rat small-for-size liver transplantation.
Augmenter of liver regeneration promotes hepatic regeneration depending on the integrity of Kupffer cell in rat small-for-size liver transplantation.
复制标题
DOI：
10.1016/j.jss.2013.02.033
复制
发表时间：
2013-08
期刊：
影响因子：
--
通讯作者：
Kang Yang;Chengyou Du;Yong Cheng;Yue Li;J. Gong;Zuojin Liu
中科院分区：
文献类型：
--
作者：
Kang Yang;Chengyou Du;Yong Cheng;Yue Li;J. Gong;Zuojin Liu
背景肝再生增强剂（ALR）可以促进肝细胞增殖，从而增强肝脏质量恢复。本研究旨在进一步探讨ALR对小体积肝移植大鼠肝再生的影响机制。方法将供体Sprague-Dawley大鼠分为库普弗细胞(KC)耗竭组(GdCl3预处理)和KC感受态组,再分为ALR亚组(门静脉输注100 μg/kg ALR)和非ALR亚组两个亚组。仅保留中叶建立小型肝移植模型。每个亚组使用 10 只大鼠进行 7 天生存研究。此外，还对核因子κB活性、再灌注损伤、残肝再生以及肿瘤坏死因子α和白细胞介素6表达水平进行了评估。结果ALR可以通过增加核因子κB活性诱导KC感受态大鼠肿瘤坏死因子α和白细胞介素6表达来加速移植物再生，从而获得更高的7 d存活率。结论ALR可以增强移植物的肝细胞增殖。小型肝移植物的效果似乎很大程度上取决于 KC 的完整性。
BackgroundAugmenter of liver regeneration (ALR) can promote hepatocyte proliferation and thereby augment liver mass restoration. This study was performed to further explore the mechanism of ALR on liver regeneration in small-for-size liver transplanted rats.MethodsDonor Sprague-Dawley rats were divided into a Kupffer cell (KC)-depleted group (pretreated with GdCl3) and KC-competent group and then further divided into two subgroups: the ALR subgroup (infused with 100 μg/kg ALR through the portal vein) and non-ALR subgroup. Only the median lobe was retained to establish the small-for-size liver transplantation model. Ten rats from each subgroup were used for the 7 d survival study. In addition, the nuclear factor κB activity, reperfusion injury, regeneration of the remnant liver, and tumor necrosis factor α and interleukin 6 expression levels were evaluated.ResultsALR could accelerate graft regeneration by increasing nuclear factor κB activity to induce tumor necrosis factor α and interleukin 6 expression in the KC-competent rats, which resulted in a higher 7 d survival rate.ConclusionsALR could enhance the hepatocellular proliferation of small-for-size liver grafts, and these effects appeared to deeply depend on the integrity of KCs.