Analysis of protoberberine alkaloids in several herbal drugs and related medicinal preparations by non-aqueous capillary electrophoresis
Analysis of protoberberine alkaloids in several herbal drugs and related medicinal preparations by non-aqueous capillary electrophoresis
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DOI：
10.1002/jssc.200401863
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发表时间：
2005-01-01
影响因子：
3.1
通讯作者：
De Hu, Z
中科院分区：
文献类型：
--
作者：
Gao, WH;Lin, SY;De Hu, Z
开发了一种简单、快速、重现性好、通用的非水毛细管电泳方法，可在 7 分钟内分离和测定三种主要活性原小檗碱生物碱，包括小檗碱、巴马汀和药根碱。研究了乙酸和电解质的浓度、有机溶剂的比例以及施加的电压对分离的影响。最佳运行缓冲液由 50 mM 乙酸铵、0.5% (v/v) 乙酸和 10% (v/v) 乙腈的甲醇溶液组成。施加的电压为18kV。通过 214 nm 的 UV 检测分析物。还研究了峰面积和分析物浓度之间的线性关系，它们在浓度范围内表现出优异的线性行为（相关系数：0.9975-0.9986）。该方法首次成功地测定了多个属中药材（黄连、小檗、黄柏、白屈菜、功劳藤）及其相关制剂中的3种生物碱，3种成分的回收率分别为小檗碱95.6-103.2%、巴马汀97.5-103.3%、巴马汀97.5-103.3%。药根碱为 96.1-103.6%。
A simple, rapid, reproducible, and universal non-aqueous capillary electrophoresis method has been developed for the separation and determination of three major active protoberberine alkaloids including berberine, palmatine, and jatrorrhizine within 7 min. The effects of the concentrations of acetic acid and electrolyte, the ratio of organic solvent, and the applied voltage on the separation were investigated. The optimum running buffer was composed of 50 mM ammonium acetate, 0.5% (v/v) acetic acid, and 10% (v/v) acetonitrile in methanol. The applied voltage was 18 kV. The analytes were detected by UV at 214 nm. The linearities between peak areas and the concentrations of the analytes were also investigated, and they exhibit excellent linear behavior over the concentration ranges (correlation coefficients: 0.9975-0.9986). The method was successfully applied to determine the three alkaloids in several families of herbal drugs (Rhizoma Coptidis, Cortex Berberidis, Cortex Phellodendri, Herba Chelidonii, Caulis Mahoniae) and their relevant medicinal preparations for the first time, and the recoveries of the three constituents ranged between 95.6-103.2% for berberine, 97.5-103.3% for palmatine, and 96.1-103.6% for jatrorrhizine.