Genetic characterization and pathogenicity assessment of highly pathogenic H5N1 avian influenza viruses isolated from migratory wild birds in 2011, South Korea
Genetic characterization and pathogenicity assessment of highly pathogenic H5N1 avian influenza viruses isolated from migratory wild birds in 2011, South Korea
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DOI：
10.1016/j.virusres.2011.07.003
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发表时间：
2011-09-01
期刊：
影响因子：
5
通讯作者：
Choi, Young Ki
中科院分区：
文献类型：
--
作者：
Kwon, Hyeok-il;Song, Min-Suk;Choi, Young Ki
高致病性H5 N1禽流感病毒在野鸟和家禽中的持续传播对人类公共卫生构成了潜在威胁。在本研究中，我们报告了高致病性禽流感H5 N1病毒（A/Md/Korea/W 401/11和A/Md/Korea/W 404/11）从候鸟粪便样品中的分离。遗传学和phlyogenetic分析表明，这些病毒是遗传相同的，拥有来自禽源病毒的基因片段，并显示出最高的序列相似性（高达99.8%），A/Ws/北海道/4/11和2009-2010蒙古样进化枝2.3.2分离株，而不是以前的韩国H5 N1病毒。这两种病毒都具有HA中的多碱基基序（QRERRK/R），但其他基因没有携带额外的毒力标记。在鸡、鸭、小鼠和雪貂中评估了A/Md/Korea/W 401/11的致病性，并与2006年进化枝2.2 HPAI H5 N1候鸟分离株（A/EM/Korea/W149/06）进行了比较。在这些宿主中的实验感染表明，这两种病毒在鸡（2.3-3.0 LD（50）s）和小鼠（3.3-3.9 LD（50）s）中具有高致病性，但A/Md/Korea/W 401/11在鸭和雪貂模型中的致病性较低。尽管两种感染病毒在上呼吸道中均恢复，但未观察到雪貂间的有效传播。这些数据表明，2011年韩国HPAI野鸟H5 N1病毒可以在哺乳动物宿主中复制，而无需预先适应，但不能维持随后的感染。这项研究强调了候鸟在高致病性禽流感H5 N1病毒在远东地区的延续和传播中的作用。随着H5 N1病毒在野生鸟类和家禽中引起的病理生物学变化，继续监测候鸟物种中的流感病毒对于有效监测高致病性或大流行性流感病毒仍然至关重要。(C)2011爱思唯尔有限公司版权所有。
The continued spread of a highly pathogenic avian influenza (HPAI) H5N1 virus among wild birds and poultry has posed a potential threat to human public health. In the present study, we report the isolation of HPAI H5N1 viruses (A/Md/Korea/W401/11 and A/Md/Korea/W404/11) from fecal samples of migratory birds. Genetic and phlyogenetic analyses demonstrated that these viruses are genetically identical possessing gene segments from avian virus origin and showing highest sequence similarities (as high as 99.8%) to A/Ws/Hokkaido/4/11 and 2009-2010 Mongolian-like clade 2.3.2 isolates rather than previous Korean H5N1 viruses. Both viruses possess the polybasic motif (QRERRRK/R) in HA but other genes did not bear additional virulence markers. Pathogenicity of A/Md/Korea/W401/11 was assessed and compared with a 2006 clade 2.2 HPAI H5N1 migratory bird isolate (A/EM/Korea/W149/06) in chickens, ducks, mice and ferrets. Experimental infection in these hosts showed that both viruses have high pathogenic potential in chickens (2.3-3.0 LD(50)s) and mice (3.3-3.9 LD(50)s), but A/Md/Korea/W401/11 was less pathogenic in duck and ferret models. Despite recovery of both infection viruses in the upper respiratory tract, efficient ferret-to-ferret transmission was not observed. These data suggest that the 2011 Korean HPAI wild bird H5N1 virus could replicate in mammalian hosts without pre-adaptation but could not sustain subsequent infection. This study highlights the role of migratory birds in the perpetuation and spread of HPAI H5N1 viruses in Far-East Asia. With the changing pathobiology caused by H5N1 viruses among wild and poultry birds, continued surveillance of influenza viruses among migratory bird species remains crucial for effective monitoring of high-pathogenicity or pandemic influenza viruses. (C) 2011 Elsevier B.V. All rights reserved.