Regulation of competence for genetic transformation in Streptococcus pneumoniae by an auto-induced peptide pheromone and a two-component regulatory system
Regulation of competence for genetic transformation in Streptococcus pneumoniae by an auto-induced peptide pheromone and a two-component regulatory system
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DOI：
10.1046/j.1365-2958.1996.501417.x
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发表时间：
1996-08-01
影响因子：
3.6
通讯作者：
Morrison, DA
中科院分区：
文献类型：
--
作者：
Pestova, EV;Havarstein, LS;Morrison, DA
肺炎链球菌遗传转化能力的调节依赖于群体感应系统，但该系统中唯一被确定其特定作用的分子元件是胞外肽信号和其输出所需的ABC转运体。在这里，我们证明了Comc的转录，该基因编码一个预测的41个残基的前体多肽，被认为是作为17个残基的成熟能力激活剂被加工和分泌的，在外源合成激活剂的反应下，它的转录比其基础表达水平增加了大约40倍，这与早期的实验一致，表明激活剂是自动催化的。我们还描述了两个新的基因comd和comm，它们编码组氨酸蛋白激酶和反应调节家族的成员，并与comc相关联。COMe的干扰既取消了对合成激活肽的反应，也取消了内源能力诱导。
The regulation of competence for genetic transformation in Streptococcus pneumoniae depends on a quorum-sensing system, but the only molecular elements of the system whose specific role have been identified are an extracellular peptide signal and an ABC-transporter required for its export. Here we show that transcription of comC, the gene encoding a predicted 41-residue precursor peptide that is thought to be processed and secreted as the 17-residue mature competence activator, increased approximately 40-fold above its basal level of expression in response to exogenous synthetic activator, consistent with earlier experiments indicating that the activator acts autocatalytically. We also describe two new genes, comD and comE, that encode members of histidine protein kinase and response-regulator families and are linked to comC. Disruption of comE abolished both response to synthetic activator peptide and endogenous competence induction.