Distinct effects of different temperatures on diapause termination, yolk morphology and MAPK phosphorylation in the silkworm, Bombyx mori.
Distinct effects of different temperatures on diapause termination, yolk morphology and MAPK phosphorylation in the silkworm, Bombyx mori.
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DOI：
10.1016/j.jinsphys.2006.08.010
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发表时间：
2006-11
影响因子：
2.2
通讯作者：
Y. Fujiwara;K. Shiomi
中科院分区：
文献类型：
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作者：
Y. Fujiwara;K. Shiomi
此前，我们发现滞育家蚕卵的冷却在获得发育能力的同时激活了卵黄细胞中的 ERK/MAPK，并且 ERK 通过激活蜕皮类固醇和山梨醇代谢关键酶基因的转录来调节滞育终止。为了进一步阐明温度信号传导和滞育终止的分子机制，分析了不同温度对滞育终止、卵黄形态以及ERK和p38 MAPK磷酸化的影响。我们证实了不同温度下滞育终止与 ERK 和 p38 MAPK 磷酸化水平变化的一致性。我们的研究还表明，不同的温度对滞育终止和卵黄形态有不同的影响，然而，这两种影响并不总是一致。我们的分析表明，对滞育终止和卵黄形态的影响可能是由 ERK 独立调节的。我们观察到温度变化可逆地改变滞育强度，磷酸化 ERK 水平的同时变化表明 ERK 可能在调节滞育强度中发挥作用。本研究表明 ERK 和 p38 MAPK 具有传递温度信号的作用，这对滞育终止很重要。
Previously, we showed that chilling of diapausing Bombyx eggs activated ERK/MAPK in yolk cells coincidentally with acquisition of developmental competence, and that ERK regulates diapause termination via activating transcription of key enzyme genes for ecdysteroid and sorbitol metabolism. To further elucidate the molecular mechanisms of temperature signaling and diapause termination, effects of different temperatures on diapause termination, yolk morphology, and ERK and p38 MAPK phosphorylation were analyzed. We confirmed the coincidence of diapause termination and changes of phosphorylated levels of ERK and p38 MAPK at various temperatures. Our study also shows that different temperatures have distinct effects on diapause termination and yolk morphology, however, these two effects did not always coincide. Our analyses suggest the possibility that the effects on diapause termination and yolk morphology are independently regulated by ERK. We observed that a temperature shift alters diapause intensity reversibly, and coincidental changes of phosphorylated ERK levels suggest that ERK may have a role in regulating the diapause intensity. The present study suggests that ERK and p38 MAPK have roles for transmitting the temperature signals that are important for diapause termination.