Dicer independent small RNAs associate with telomeric heterochromatin
Dicer independent small RNAs associate with telomeric heterochromatin
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DOI：
10.1261/rna.1423309
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发表时间：
2009-07-01
期刊：
影响因子：
4.5
通讯作者：
Dou, Yali
中科院分区：
文献类型：
--
作者：
Cao, Fang;Li, Xiangzhi;Dou, Yali
小RNA在异染色质结构的建立和维持中起着重要作用。我们发现在小鼠胚胎干细胞中存在端粒特异的小RNA(tel-sRNAs)，它的长度类似于24个核苷酸，不依赖于DICER，在3‘端有2’-O-甲基化。TelsRNA是不对称的，对富含端粒G的链具有特异性，并且从原生动物到哺乳动物细胞在进化上都是保守的。此外，在携带H3K4甲基转移酶MLL((-/-))零突变的细胞中，tel-sRNA上调，在携带组蛋白H3K9甲基转移酶SUV39H(SUV39H1/H2(-/-))零突变的细胞中下调，表明它们受到表观遗传调控。这些结果支持tel-sRNA是异染色质相关的pi样小RNA。
Small RNAs play important roles in the establishment and maintenance of heterochromatin structures. We show the presence of telomere specific small RNAs (tel-sRNAs) in mouse embryonic stem cells that are similar to 24 nucleotides in length, Dicer-independent, and 2'-O-methylated at the 3' terminus. The tel-sRNAs are asymmetric with specificity toward telomere G-rich strand, and evolutionarily conserved from protozoan to mammalian cells. Furthermore, tel-sRNAs are up-regulated in cells that carry null mutation of H3K4 methyltransferase MLL (Mll((-/-))) and down-regulated in cells that carry null mutations of histone H3K9 methyltransferase SUV39H (Suv39h1/h2((-/-))), suggesting that they are subject to epigenetic regulation. These results support that tel-sRNAs are heterochromatin associated pi-like small RNAs.