Probing the alpha-sarcin region of Escherichia coli 23S rRNA with a cDNA oligomer.
Probing the alpha-sarcin region of Escherichia coli 23S rRNA with a cDNA oligomer.
复制标题
使用 cDNA 寡聚物探测大肠杆菌 23S rRNA 的 α-sarcin 区域。
DOI：
10.1093/nar/16.22.10817
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发表时间：
1988
影响因子：
14.9
通讯作者：
Hill,WE
中科院分区：
文献类型：
--
作者：
White,GA;Wood,T;Hill,WE
将人尿激酶（uPA）基因的5 '侧翼区域与报告基因氯霉素乙酰转移酶（CAT）基因融合，并通过转染两种人细胞系检测其活性。从5 ‘端逐渐缺失的uPA调控区维持了高水平的表达，前提是至少保留了5 ’侧区域的1870个（在A1251细胞中）或1963个（在hfs10细胞中）核苷酸。从-2350到-1824的DNA片段具有增强子特性，刺激无增强子的SV40早期启动子的转录，而不依赖于取向和距离。内部缺失仍然保留增强子元件，表明-1824和-1572之间存在负顺式作用序列。事实上，它们的去除会增加uPA的转录活性。uPA-CAT融合基因在两种细胞系中的表达差异也被观察到，表明存在细胞特异性顺式作用序列。
The 5′ flanking region of the human urokinase (uPA) gene has been fused to the reporter chloramphenicol acetyl transferase (CAT) gene and its activity assayed by transfection in two human cell lines. Progressive deletions of the uPA regulatory region from the 5′ end maintain a high level of expression provided at least 1870 (in A1251 cells) or 1963 (in HFS10cells) nucleotides of the 5′ flanking region are retained. A DNA fragment from -2350 to -1824 has enhancer properties, stimulating transcription of an enhancerless SV40 early promoter independently of orientation and distance. Internal deletions of that still retain the enhancer element reveal the presence of negative cis-acting sequences between -1824 and -1572. Their removal, in fact, increases uPA transcriptional activity. Differences ofexpression of the uPA-CAT fusion genes in the two cell lines are also observed, indicating the presence of cell-specific cis-acting sequences.