Surface Modification with Micropatterned Heparin/Poly-L-Lysine Nanoparticles to Direct Platelet and Endothelial Cell Behavior
Surface Modification with Micropatterned Heparin/Poly-L-Lysine Nanoparticles to Direct Platelet and Endothelial Cell Behavior
复制标题
用微图案化肝素/聚-L-赖氨酸纳米颗粒进行表面修饰以指导血小板和内皮细胞行为
DOI：
10.1166/jbt.2017.1661
复制
发表时间：
2017-10
影响因子：
0.1
通讯作者：
Wang Qingmei
中科院分区：
文献类型：
--
作者：
Liu Tao;Li Xia;Liu Shihui;Tang Xiaohan;Wang Xin;Gong Tao;Hu Youdong;Ding Hongyan;Luo Xun;Pan Changjiang;Wang Qingmei
支架植入术后血栓和再狭窄仍然是一个问题。通过加入功能分子对血管支架进行表面修饰以提高其生物相容性是近年来研究的热点。然而，到目前为止，研究人员还没有找到表面物理化学性质和结构的正确组合来满足临床需求。为了研究有序结构和生物功能对血小板和血管细胞行为的影响，本研究采用微接触打印技术在316L不锈钢表面构建了3种平行的肝素/聚赖氨酸纳米颗粒凹槽（25/25 μm， 50/50 μm和100/100 μm）。傅里叶变换红外和扫描电镜结果表明，纳米颗粒成功地固定在多巴胺包被表面。水接触角和生物分子定量表征结果表明，三个模式基团具有相似的亲水性，以及肝素和胺的密度。体外血液相容性评价结果表明，纳米颗粒表面可选择性地阻止血小板粘附。此外，发现图案区域抑制内皮细胞粘附。然而，当培养基中含有高浓度的肝素结合蛋白时，内皮细胞在图案区域的生长情况大大改善。
Thrombus and restenosis after stent implantation continue to be problematic. Surface modification by incorporating functional molecules to improve the biocompatibility of vascular stent has been a hot-topic in recent study. To date, however, researchers have not yet found the right combination of surface physicochemical properties and architecture to meet clinical requirement. To investigate the influence of ordered structure and biofunction on platelets and vascular cells behavior, in this study, we construct three kinds of parallel heparin/poly-l-lysine nanoparticles grooves (25/25 μm, 50/50 μm and 100/100 μm) on dopamine-coated 316L stainless steel surface by using micro-contact printing method. Fourier transform infrared and scanning electron microscopy results demonstrated that the nanoparticles were successfully immobilized to dopamine-coated surface. Water contact angle and biomolecules quantitative characterization results suggested that three patterned groups were of similar hydrophilicity, as well as heparin and amine density. In vitro blood compatibility evaluation result indicated that surface patterned with nanoparticles may selectively prevent platelet adhesion. Besides, the patterned area was found to inhibit endothelial cell adhesion. However, when culture medium contains high concentration of heparin-binding proteins, the endothelial cell growth profile on patterned area was greatly improved.