RNA-sequence analysis of gene expression from honeybees (Apis mellifera) infected with Nosema ceranae.
RNA-sequence analysis of gene expression from honeybees (Apis mellifera) infected with Nosema ceranae.
复制标题
DOI：
10.1371/journal.pone.0173438
复制
发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Botti S
中科院分区：
文献类型：
--
作者：
Badaoui B;Fougeroux A;Petit F;Anselmo A;Gorni C;Cucurachi M;Cersini A;Granato A;Cardeti G;Formato G;Mutinelli F;Giuffra E;Williams JL;Botti S
蜜蜂经常受到包括寄生虫在内的多种生物应激源的影响。这项研究检测了感染中华微孢子虫(N.ceranae)的蜜蜂。中华绒毛虫感染增加了蜜蜂的能量需求，并可能导致它们的存活率下降。RNA-seq法检测感染后第5、10、15天(P.I)的基因表达。基因的表达水平、异构体、选择性转录起始点(TSS)和差异启动子的使用揭示了转录和转录后基因调控的复杂模式，表明蜜蜂使用一系列策略来应对中华绒毛虫感染的压力。中华绒毛虫感染可通过以下方式导致蜜蜂免疫功能下降：(1)干扰宿主氨基酸代谢；(2)下调抗菌肽的表达；(3)下调角质层的表达；(4)下调气味结合蛋白的表达。
Honeybees (Apis mellifera) are constantly subjected to many biotic stressors including parasites. This study examined honeybees infected with Nosema ceranae (N. ceranae). N. ceranae infection increases the bees energy requirements and may contribute to their decreased survival. RNA-seq was used to investigate gene expression at days 5, 10 and 15 Post Infection (P.I) with N. ceranae. The expression levels of genes, isoforms, alternative transcription start sites (TSS) and differential promoter usage revealed a complex pattern of transcriptional and post-transcriptional gene regulation suggesting that bees use a range of tactics to cope with the stress of N. ceranae infection. N. ceranae infection may cause reduced immune function in the bees by: (i)disturbing the host amino acids metabolism (ii) down-regulating expression of antimicrobial peptides (iii) down-regulation of cuticle coatings and (iv) down-regulation of odorant binding proteins.