Effects of CPEB1 in the anterior cingulate cortex on visceral pain in mice
Effects of CPEB1 in the anterior cingulate cortex on visceral pain in mice
复制标题
前扣带皮层 CPEB1 对小鼠内脏痛的影响
DOI：
10.1016/j.brainres.2019.02.001
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发表时间：
2019-06
期刊：
影响因子：
--
通讯作者：
Liu S. B.
中科院分区：
文献类型：
--
作者：
Wang X. S;Yue J;Hu L. N;Tian Z;Yang L. K;Lu L;Zhao M. G;Liu S. B.
肠易激综合征患者患有慢性内脏疼痛，其中一些患者伴有焦虑共病。胞质多聚腺苷酸化元件结合蛋白1（CPEB 1）介导mRNA的胞质多聚腺苷酸化并促进其翻译。我们先前的研究表明，在炎症性疼痛的小鼠模型中，杏仁核中的CPEB 1敲低产生抗焦虑作用，但不产生镇痛作用。然而，CPEB 1在前扣带皮层（ACC）内脏痛调制中的作用仍不清楚。本研究采用小鼠结肠内注射酵母多糖的方法，建立小鼠内脏痛模型。酵母多糖注射显著诱导小鼠内脏疼痛和焦虑样行为，并增加ACC中GluA 1、S845和S831磷酸化GluA 1和CPEB 1的水平。通过AAV-CPEB 1-shRNA敲低ACC中的CPEB 1可减少酵母多糖诱导的小鼠疼痛和焦虑样行为。这一观察结果与AMPA受体、突触素和PSD 95水平降低密切相关。这些数据表明，ACC中的CPEB 1是内脏疼痛和焦虑合并症的潜在治疗靶点。
Patients with irritable bowel syndrome suffer from chronic visceral pain, and in some of them, this is accompanied by anxiety comorbidity. Cytoplasmic polyadenylation element binding protein 1 (CPEB1) mediates the cytoplasmic polyadenylation of mRNAs and facilitates their translation. Our previous studies have shown that CPEB1 knockdown in the amygdala exerts anxiolytic but not analgesic effects in a mouse model of inflammatory pain. However, the roles of CPEB1 in the anterior cingulate cortex (ACC) in visceral pain modulation remain unclear. In this study, a visceral pain mouse model was established by injecting zymosan into the colon of mice. Zymosan injection significantly induced visceral pain- and anxiety-like behaviors in mice and increased the levels of GluA1, phosphorylated GluA1 at S845 and S831, and CPEB1 in the ACC. CPEB1 knockdown in the ACC by AAV-CPEB1-shRNA reduced zymosan-induced pain- and anxiety-like behaviors in mice. This observation was closely correlated with reduced AMPA receptor, synaptophysin, and PSD95 levels. These data suggest that CPEB1 in the ACC is a potential therapeutic target for visceral pain and anxiety comorbidity.
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DOI：
10.1523/jneurosci.5138-11.2012
发表时间：
2012-02-08
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Bogen O;Alessandri-Haber N;Chu C;Gear RW;Levine JD
通讯作者：
Levine JD
DOI：
10.1098/rstb.2013.0146
发表时间：
2014-01-05
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Zhuo M
通讯作者：
Zhuo M
影响因子：
4.8
作者：
Kwon M;Han J;Kim UJ;Cha M;Um SW;Bai SJ;Hong SK;Lee BH
通讯作者：
Lee BH
慢性内脏痛小鼠模型中皮质谷氨酸能 AMPA 受体的长期上调
影响因子：
3.6
作者：
Liu SB;Zhang MM;Cheng LF;Shi J;Lu JS;Zhuo M
通讯作者：
Zhuo M
DOI：
10.1016/j.bbrc.2011.05.065
发表时间：
2011-06-17
影响因子：
3.1
作者：
Kim, Ki Chan;Joo, So Hyun;Shin, Chan Young
通讯作者：
Shin, Chan Young