SPARE GONADOTROPIN RECEPTORS IN RAT TESTIS
SPARE GONADOTROPIN RECEPTORS IN RAT TESTIS
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DOI：
10.1038/newbio244219a0
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发表时间：
1973-01-01
期刊：
影响因子：
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通讯作者：
DUFAU, ML
中科院分区：
文献类型：
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作者：
CATT, KJ;DUFAU, ML
在大鼠睾丸的颗粒和可溶性间质细胞组分中已证实促性腺激素受体位点对促黄体生成素（LH）和人绒毛膜促性腺激素（HCG）具有高特异性和亲和力1 -4。这种激素结合位点已用于LH和HCG的放射性配体受体测定1，2，以及促性腺激素与靶组织结合的结构决定簇的研究5 -7。在用HCG孵育完整大鼠睾丸的过程中，通过放射免疫测定法检测了体外促性腺激素刺激过程中产生的环腺苷酸和睾酮的促性腺激素作用的其他参数8 -11。去囊完整大鼠睾丸在体外对促性腺激素刺激高度敏感，对低至0.1 ng ml−1（10− 12 M）的HCG浓度有反应，合成和释放睾酮，对高浓度有反应，合成和释放环AMP 10。通过同时测量14 C-腺嘌呤掺入和环AMP放射免疫测定，表明在与HCG孵育期间环AMP的释放反映了环AMP的合成9，10。
GONADOTROPHIN receptor sites with high specificity and affinity for luteinizing hormone (LH) and human chorionic gonadotrophin (HCG) have been demonstrated in particulate and soluble interstitial cell fractions of rat testis1–4. Such hormone binding sites have been used in radioligand receptor assays for LH and HCG1,2, and in studies on the structural determinants of gonadotrophin binding to target tissue5–7. Other parameters of gonadotrophin action have been examined during incubation of intact rat testes with HCG by radioimmunoassay of the cyclic AMP and testosterone produced during trophic hormone stimulationin vitro8–11. Decapsulated intact rat testes are highly sensitive to gonadotrophic stimulationin vitro, responding to HCG concentrations as low as 0.1 ng ml−1(10−12M) with synthesis and release of testosterone and to higher concentrations with synthesis and release of cyclic AMP10. Release of cyclic AMP during incubation with HCG has been shown to reflect cyclic AMP synthesis by simultaneous measurement of14C-adenine incorporation and radioimmunoassay of cyclic AMP9,10.