Role of extracellular matrix in wound repair by cultured gastric mucosal cells.
Role of extracellular matrix in wound repair by cultured gastric mucosal cells.
复制标题
细胞外基质在培养胃粘膜细胞伤口修复中的作用。
DOI：
--
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
N. Sato
中科院分区：
文献类型：
--
作者：
H. Mikami;S. Watanabe;M. Hirose;N. Sato
观察细胞外基质对胃上皮细胞损伤修复的影响。人工创面是在不同类型的细胞外基质(如I型和IV型胶原、层粘连蛋白、纤维连接蛋白和Matrigel)上形成的融合兔胃上皮细胞片上机械剥离细胞制成的。定量分析伤口大小的变化。在创面修复过程中，观察到细胞的迁移和增殖。不同细胞外基质的伤口修复速度不同，Matrigel的修复速度最快。这项研究中使用的细胞外基质类型既调节细胞迁移又调节细胞增殖。因此，细胞外基质在胃粘膜创面愈合率中起重要作用。
Effects of extracellular matrix on wound repair of cultured gastric epithelial cells were assessed. Artificial wounds were made by mechanical cell denudation in confluent rabbit gastric epithelial cell sheets which were formed on different types of extracellular matrix (e.g., collagen type I and type IV, laminin, fibronectin and Matrigel). Changes in wound size were analyzed quantitatively. Cell migration and proliferation were observed in stages of the wound repair process. The speed of wound repair was different with each extracellular matrix studied and was fastest on Matrigel. The type of extracellular matrix used in this study modulated both cell migration and proliferation. Therefore, it is concluded that extracellular matrix plays an important role in rates of gastric mucosal wound healing.