Flow cytometric analysis of de novo acute myeloid leukemia in childhood: report from the Japanese Pediatric Leukemia/Lymphoma Study Group
Flow cytometric analysis of de novo acute myeloid leukemia in childhood: report from the Japanese Pediatric Leukemia/Lymphoma Study Group
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DOI：
10.1007/s12185-010-0754-y
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发表时间：
2011-01-01
影响因子：
2.1
通讯作者：
Komada, Yoshihiro
中科院分区：
文献类型：
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作者：
Ohta, Hideaki;Iwamoto, Shotaro;Komada, Yoshihiro
M0（未分化的急性髓性白血病，n= 11），M1（急性髓细胞白血病伴低分化，n= 41），M2（急性髓细胞白血病伴分化，n= 113）、M4（急性髓单核细胞白血病，n= 47）、M5（急性单核细胞白血病，n= 54）、M6（急性红白血病，n= 6）和M7（急性巨核细胞白血病，n= 61）。骨髓或外周血样品的单核细胞用针对以下抗原的荧光素异硫氰酸酯（FITC）和藻红蛋白（PE）标记的单克隆抗体的各种组合染色：CD 4、CD 7、CD 13、CD 14、CD 15、CD 19、CD 33、CD 34、CD 36、CD 41、CD 42 b、CD 45、CD 56、CD 61、CD 65、CD 117、血型糖蛋白A（GPA：CD235a）和HLA-DR。通过收集10，000个非门控列表模式事件进行双色流式细胞术免疫表型分析。抗原被认为是
M0 (acute myeloid leukemia without differentiation, n= 11), M1 (acute myelocytic leukemia with little differentiation, n= 41), M2 (acute myelocytic leukemia with differentiation, n= 113), M4 (acute myelomonocytic leukemia, n= 47), M5 (acute monocytic leukemia, n= 54), M6 (acute erythroleukemia, n= 6), and M7 (acute megakaryoblastic leukemia, n= 61). Mononuclear cells of bone marrow or peripheral blood samples were stained with various combinations of fluorescein isothiocyanate (FITC)-and phycoerythrin (PE)-labeled monoclonal antibodies against the following antigens: CD4, CD7, CD13, CD14, CD15, CD19, CD33, CD34, CD36, CD41, CD42b, CD45, CD56, CD61, CD65, CD117, glycophorin A (GPA: CD235a), and HLA-DR. Cytoplasmic MPO was also detected by anti-MPO antibody after permeabilization. Two-color flow cytometric immunophenotyping was performed by collecting 10,000 ungated list mode events. An antigen was considered as