An autonomous DNA nanomachine maps spatiotemporal pH changes in a multicellular living organism
An autonomous DNA nanomachine maps spatiotemporal pH changes in a multicellular living organism
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DOI：
10.1038/ncomms1340
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发表时间：
2011-06-01
影响因子：
16.6
通讯作者：
Krishnan, Yamuna
中科院分区：
文献类型：
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作者：
Surana, Sunaina;Bhat, Jaffar M.;Krishnan, Yamuna
结构DNA纳米技术旨在从DNA构建合成分子机器。DNA纳米机器是人工设计的组件，可以响应外部提示在定义的构象之间切换。虽然已经证明可以制造出DNA机器和基本的步行器，但这种基于DNA的自主分子装置的功能尚未在生物体内实现。在这里，我们展示了一个pH值触发的DNA纳米机器内线虫秀丽隐杆线虫的操作。纳米机器使用荧光共振能量转移来有效地映射与野生型以及突变蠕虫中的内吞作用相关的时空pH变化，从而在各种遗传背景中展示了生物体环境中的自主功能。从这第一次演示的DNA纳米机器在体内的独立功能，我们观察到，合理设计的DNA为基础的分子装置保留其在体外的功能与定量精度。这将DNA纳米器件定位为询问复杂生物现象的令人兴奋和强大的工具。
Structural DNA nanotechnology seeks to build synthetic molecular machinery from DNA. DNA nanomachines are artificially designed assemblies that switch between defined conformations in response to an external cue. Though it has proved possible to create DNA machines and rudimentary walkers, the function of such autonomous DNA-based molecular devices has not yet been achieved inside living organisms. Here we demonstrate the operation of a pH-triggered DNA nanomachine inside the nematode Caenorhabditis elegans. The nanomachine uses fluorescence resonance energy transfer to effectively map spatiotemporal pH changes associated with endocytosis in wild type as well as mutant worms, demonstrating autonomous function within the organismal milieu in a variety of genetic backgrounds. From this first demonstration of the independent functionality of a DNA nanomachine in vivo, we observe that rationally designed DNA-based molecular devices retain their in vitro functionality with quantitative precision. This positions DNA nanodevices as exciting and powerful tools to interrogate complex biological phenomena.