QUANTITATIVE ELECTROPHORESIS IN POLYACRYLAMIDE GELS OF 2-40 PERCENT
QUANTITATIVE ELECTROPHORESIS IN POLYACRYLAMIDE GELS OF 2-40 PERCENT
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DOI：
10.1016/s0021-9673(00)92958-3
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发表时间：
1972-01-01
期刊：
影响因子：
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通讯作者：
GARNER, F
中科院分区：
文献类型：
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作者：
BLATTLER, DP;BRADLEY, A;GARNER, F
丙烯酰胺的浓度、交联剂双丙烯酰胺的浓度和聚合条件影响聚丙烯酰胺凝胶的结构及其作为分子过滤器的适用性。有证据表明，丙烯酰胺浓度的变化往往会影响凝胶的平均“孔”的大小，而双丙烯酰胺浓度的变化往往会影响最大的“孔”的大小。证据表明，双丙烯酰胺可以作为链终止剂。提供了从2%丙烯酰胺到40%丙烯酰胺凝胶制备优良分子过滤器的说明。
The concentration of acrylamide, the concentration of the crosslinking agent bisacrylamide, and the polymerization conditions affect the structure of polyacrylamide gels and their suitability as molecular filters. Evidence is presented that a change in acrylamide concentration tends to affect the average “pore” size of the gel while changes in bisacrylamide concentration tends to affect the maximum “pore” size. Evidence is presented that bisacrylamide can act as a chain termination agent. Instructions are provided for preparing excellent molecular filters from 2% acrylamide to 40% acrylamide gels.