A trial of phenome analysis using 4000 Ds-insertional mutants in gene-coding regions of Arabidopsis
A trial of phenome analysis using 4000 Ds-insertional mutants in gene-coding regions of Arabidopsis
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DOI：
10.1111/j.1365-313x.2006.02808.x
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发表时间：
2006-08-01
期刊：
影响因子：
7.2
通讯作者：
Shinozaki, Kazuo
中科院分区：
文献类型：
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作者：
Kuromori, Takashi;Wada, Takuji;Shinozaki, Kazuo
覆盖所有拟南芥基因的突变系使我们能够追求系统的功能基因组学。一个全面的表型描述，称为表型组，是高度追求后，在分析组学数据。我们选择了4000个转座子插入的转座子插入株系，并系统地观察了每个株系的可见表型。对于萌发后的前3周，使植物在琼脂平板上生长，并记录幼龄表型。然后将植物转移到土壤中，并在每个生长阶段记录它们的表型。约140个株系表现出清晰和可重复的可见表型，包括新的表型突变体以及以前报道的。将所有表现出可见表型的突变体的描述分为8个主要类别（幼苗、叶片、开花和生长、茎、分枝、花、角果和种子产量）和43个次要类别的形态表型。表型图像已输入可检索的数据库（http：//rarge.gsc.riken.jp/phenome/）。通过使用多个等位基因研究的一个例子是与甘油脂生物合成相关的新基因的突变体，其具有独特的可见的萼片开放表型。我们的研究结果表明，我们可以发现更多的新的可见表型及其相应的基因，基因敲除的表型突变体还没有穷尽。本研究为植物基因敲除系的大规模表型分析提供了基础数据，并将有助于完成一项国际努力，即建立一个包含拟南芥所有功能基因的表型数据库。
Mutant lines covering all Arabidopsis genes allow us to pursue systematic functional genomics. A comprehensive phenotype description, called a phenome, is highly sought after in the profiling of -omics data. We selected 4000 transposon-insertional lines with transposon insertions in their gene-coding regions, and systematically observed the visible phenotype of each line. For the first 3 weeks after germination, plants were grown on agar plates and the juvenile phenotypes were recorded. Then the plants were transferred to soil and their phenotypes were recorded at each growth stage. About 140 lines showed clear and reproducible visible phenotypes, including novel phenotypic mutants as well as previously reported ones. All descriptions of the mutants showing visible phenotypes were classified into eight primary categories (seedling, leaves, flowering and growth, stems, branching, flowers, siliques and seed yield) and 43 secondary categories of morphological phenotypes. Phenotypic images have been entered into a searchable database (http://rarge.gsc.riken.jp/phenome/). One example investigated through the use of plural alleles was a mutant of a novel gene related to glycerolipid biosynthesis, with a unique visible phenotype of sepal opening. Our results suggest that we can find more novel visible phenotypes and their corresponding genes, and that phenotypic mutants of gene knockouts are not exhausted yet. This study provides basic data on large-scale phenotyping of gene knockout lines in plants, and will contribute to the completion of an international effort to develop a phenome database of all the functional genes in Arabidopsis.