The discovery of bacterial biofilm in patients with muscle invasive bladder cancer
The discovery of bacterial biofilm in patients with muscle invasive bladder cancer
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DOI：
10.1111/apm.13097
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发表时间：
2021-02-26
期刊：
影响因子：
2.8
通讯作者：
Azawi, Nessn
中科院分区：
文献类型：
--
作者：
Nadler, Naomi;Kvich, Lasse;Azawi, Nessn
在多种癌症类型中发现了微生物的致癌作用。在泌尿科，膀胱鳞状细胞癌的发展，由于寄生虫感染曼氏血吸虫被广泛接受。生物膜的致癌潜力已经在结直肠癌中进行了研究，并且实验研究表明，细菌如大肠杆菌驱动结直肠癌的发展。值得注意的是，大肠杆菌是所有尿路感染的80%的原因。最近的研究结果表明，与健康受试者相比，膀胱癌患者的尿液微生物组发生了变化。在这个病例系列中，我们展示了我们在人类膀胱癌组织中生物膜形成的发现。从丹麦国家生物库中获得了10名接受常规经尿道膀胱肿瘤切除术（TURBT）的患者的组织样本。通过荧光原位杂交检测病理组织中细菌聚集体的存在。在10名患者中的2名中，分析显示在表面上皮上有大量细菌聚集。两例阳性病例均为pT2尿路上皮膀胱癌。我们的研究结果表明，生物膜发生在尿路上皮癌组织表明生物膜形成和膀胱癌之间的关联。
The carcinogenic effects of microorganisms have been discovered in multiple cancer types. In urology, the development of squamous cell carcinoma of the bladder due to the parasitic infection with Schistosoma Mansoni is widely accepted. The oncogenic potential of biofilms has been studied in colorectal cancer and experimental studies have shown that bacteria such as Escherichia coli drive the development of colorectal cancer. Notably, Escherichia coli is responsible for 80% of all urinary tract infections. Recent findings suggest an altered urinary microbiome in patients with bladder cancer compared to healthy subjects. In this case series, we demonstrate our findings of biofilm formation in human bladder cancer tissue. Tissue samples from ten patients that underwent routine Transurethral Resection of Bladder Tumor (TURBT) were obtained from the Danish National Biobank. Pathological tissue was examined for presence of bacterial aggregates by Fluorescence in situ Hybridization. In two of ten patients, analysis showed abundant bacterial aggregation on the surface epithelium. Both positive cases had pT2 urothelial bladder cancer. Our findings suggest that biofilm occurs in urothelial cancer tissue indicating an association between biofilm formation and bladder cancer.