Effects of hypoxia on leukotriene activity and vasomotor tone in isolated sheep lungs.
Effects of hypoxia on leukotriene activity and vasomotor tone in isolated sheep lungs.
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缺氧对离体羊肺白三烯活性和血管舒缩张力的影响。
DOI：
10.1152/jappl.1990.68.6.2457
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Sylvester,JT
中科院分区：
文献类型：
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作者：
Schnader,J;Undem,B;Adams3rd,GK;Peters,SP;Adkinson,NF;Sylvester,JT
为了确定肺缺氧血管收缩是否与肺中硫多肽白三烯（SPLT）的释放有关，我们采用生物测定法（豚鼠回肠）和放射免疫测定法测定了羊肺（n = 20）分离的淋巴、灌注液和支气管肺泡灌洗液（BAL）中SPLT的活性，这些液用恒定流量的自体血（100 ml.kg-1）原位灌注。Min-1)含吲哚美辛（60微克/毫升）。该方案包括三个阶段，每个阶段至少持续1小时。实验肺吸入O2浓度（FIO2）在第1期和第3期为28.2%，第2期为4.2%。在对照肺中，整个FIO2为28.2%。缺氧使肺动脉压升高，但未改变每个时段最后30分钟测量的气管峰值压、肺淋巴流量或体重增加。SPLT活性在肺淋巴中最高，在BAL液中最低。缺氧不改变任何液体的SPLT活性。未使用吲哚美辛治疗的肺也获得了类似的结果（n = 15）。这些数据不支持缺氧肺血管收缩是由splt介导的假设。
To determine whether hypoxic pulmonary vasoconstriction was associated with release of sulfidopeptide leukotrienes (SPLTs) from the lung, we measured SPLT activity by bioassay (guinea pig ileum) and radioimmunoassay in lymph, perfusate, and bronchoalveolar lavage (BAL) fluid from sheep lungs (n = 20) isolated and perfused in situ with a constant flow of autologous blood (100 ml.kg-1.min-1) containing indomethacin (60 micrograms/ml). The protocol consisted of three periods, each at least 1 h in duration. In experimental lungs, inspired O2 concentration (FIO2) was 28.2% in periods 1 and 3 and 4.2% in period 2. In control lungs, FIO2 was 28.2% throughout. Hypoxia increased pulmonary arterial pressure but did not alter peak tracheal pressure, lung lymph flow, or weight gain measured during the last 30 min of each period. SPLT activity was greatest in lung lymph and least in BAL fluid. Hypoxia did not alter SPLT activity in any fluid. Similar results were obtained in lungs not treated with indomethacin (n = 15). These data do not support the hypothesis that hypoxic pulmonary vasoconstriction is mediated by SPLTs.