The Relationship between Environmental Dioxygen and Iron-Sulfur Proteins Explored at the Genome Level.
The Relationship between Environmental Dioxygen and Iron-Sulfur Proteins Explored at the Genome Level.
复制标题
在基因组水平探索的环境二氧化物与铁硫蛋白之间的关系。
DOI：
10.1371/journal.pone.0171279
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Banci L
中科院分区：
文献类型：
--
作者：
Andreini C;Rosato A;Banci L
大约20亿年前，由于光合生物产生的分子氧的积累，地球的大气层经历了巨大的变化。这种转变导致铁生物利用度降低，同时使生物体暴露于氧化应激的威胁。铁-硫（Fe-S）簇需要铁离子用于其生物合成，并且如果暴露于活性氧物种则是不稳定的。为了评估上述转变如何影响生物体对Fe-S簇的利用，我们比较了400多种原核生物基因组编码的Fe-S蛋白的分布与其分子氧需求的函数关系。好氧生物比大多数具有相似基因组大小的厌氧生物使用更少的Fe-S蛋白。此外，需氧菌已经进化出特异性的Fe-S蛋白，其结合铁需求较少且化学上更稳定的Fe 2S 2簇，同时减少其基因组中Fe 4S 4结合蛋白的数量。然而，有一个共同的核心Fe-S蛋白家族主要由Fe 4S 4结合蛋白组成。这些家族的成员也存在于人类中。人Fe-S蛋白在细胞隔室内的分布表明，线粒体蛋白遗传自需氧菌的原核蛋白，而细胞核和细胞质Fe-S蛋白遗传自厌氧生物。
About 2 billion years ago, the atmosphere of the Earth experienced a great change due to the buildup of dioxygen produced by photosynthetic organisms. This transition caused a reduction of iron bioavailability and at the same time exposed living organisms to the threat of oxidative stress. Iron-sulfur (Fe-S) clusters require iron ions for their biosynthesis and are labile if exposed to reactive oxygen species. To assess how the above transition influenced the usage of Fe-S clusters by organisms, we compared the distribution of the Fe-S proteins encoded by the genomes of more than 400 prokaryotic organisms as a function of their dioxygen requirements. Aerobic organisms use less Fe-S proteins than the majority of anaerobic organisms with a similar genome size. Furthermore, aerobes have evolved specific Fe-S proteins that bind the less iron-demanding and more chemically stable Fe2S2 clusters while reducing the number of Fe4S4-binding proteins in their genomes. However, there is a shared core of Fe-S protein families composed mainly by Fe4S4-binding proteins. Members of these families are present also in humans. The distribution of human Fe-S proteins within cell compartments shows that mitochondrial proteins are inherited from prokaryotic proteins of aerobes, whereas nuclear and cytoplasmic Fe-S proteins are inherited from anaerobic organisms.