Electrospun membranes of PELCL/PCL-REDV loading with miRNA-126 for enhancement of vascular endothelial cell adhesion and proliferation.
Electrospun membranes of PELCL/PCL-REDV loading with miRNA-126 for enhancement of vascular endothelial cell adhesion and proliferation.
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DOI：
10.1016/j.msec.2017.12.005
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发表时间：
2018-04
期刊：
影响因子：
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通讯作者：
Fang Zhou;Meiling Wen;Peiqiong Zhou;Yunhui Zhao;Xiaoling Jia;Yubo Fan;Xiaoyan Yuan
中科院分区：
文献类型：
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作者：
Fang Zhou;Meiling Wen;Peiqiong Zhou;Yunhui Zhao;Xiaoling Jia;Yubo Fan;Xiaoyan Yuan
血管生物材料表面修饰快速内皮化是预防血栓形成和内膜增生的重要途径。此外，治疗性操纵microrna （miRNA）表达，通过电纺丝超细纤维局部递送miRNA模拟物或抑制剂，已经证明了组织再生的前景。在这项工作中，通过结合Arg-Glu-Asp-Val （REDV）肽修饰纤维表面，开发了一种双功能的电纺丝膜，以增强血管内皮细胞（VEC）的粘附，并在电纺丝纤维中包封miRNA-126 （miR-126）复合物，加速VEC的增殖。采用聚乙二醇-b-聚（l-乳酸-co-ε-己内酯）（PELCL）和端接redv的聚己内酯（PCL）（质量比为50/50）的乳液静电纺丝方法制备了静电纺丝膜，其中miR-126通过aredv肽修饰的三甲基壳聚糖-g-聚乙二醇包封。通过引入低分子量的端接REDV的PCL，得到的静电纺丝纤维可以在其表面被REDV修饰，也可以实现相对快速的miR-126释放谱，有利于VEC增殖。结果表明，在电纺丝膜上直接播种VECs可增强细胞的粘附和增殖能力。电纺丝纤维膜的REDV肽修饰与可控miRNA释放相结合，可能提供表面引导和生化信号的协同策略，以支持和调节VECs对血管组织再生的影响。
Surface modification for rapid endothelialization of vascular biomaterials is known as an important way to prevent thrombosis and intimal hyperplasia. Moreover, therapeutical manipulation of microRNAs (miRNAs) expressionvialocal delivery of miRNA mimics or inhibitors by electrospun ultrafine fibers has demonstrated the promise in tissue regeneration. In this work, a dual-functional electrospun membrane was developed by combining Arg-Glu-Asp-Val (REDV) peptide-modification of the fiber surface to enhance vascular endothelial cell (VEC) adhesion and encapsulation of miRNA-126 (miR-126) complexes in the electrospun fibers to accelerate VEC proliferation. The electrospun membranes were specially prepared by emulsion electrospinning of poly(ethylene glycol)-b-poly(l-lactide-co-ε-caprolactone) (PELCL) and REDV-terminated polycaprolactone (PCL) (50/50 mass ratio), in which miR-126 was encapsulatedviaREDV peptide-modified trimethyl chitosan-g-poly(ethylene glycol). By introduction of REDV-terminated PCL with lower molecular weight, the obtained electrospun fibers could be modified by REDV on their surface, and also achieve a relatively fast release profile of miR-126 in favor of VEC proliferation. Results of direct seeding VECs on the electrospun membranes indicated the enhanced cell adhesion and proliferation. The combination of REDV peptide-modification of the electrospun fibrous membranes and controllable miRNA release may provide a synergistic strategy of surface guidance and biochemical signals to support and modulate VECs for vascular tissue regeneration.