INDUCTION OF HUMAN TISSUE-PLASMINOGEN ACTIVATOR IN THE MAMMARY-GLAND OF TRANSGENIC GOATS
INDUCTION OF HUMAN TISSUE-PLASMINOGEN ACTIVATOR IN THE MAMMARY-GLAND OF TRANSGENIC GOATS
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DOI：
10.1038/nbt0794-699
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发表时间：
1994-07-01
期刊：
影响因子：
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通讯作者：
ROBERTS, B
中科院分区：
文献类型：
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作者：
EBERT, KM;DITULLIO, P;ROBERTS, B
来自第一代转基因雄性的三只转基因雌性在11到12个月龄之间使用一系列的雌激素和孕激素注射来诱导乳汁分泌。牛奶中含有与最初的创始女性相同浓度的人长效组织纤溶酶原激活剂(LAtPA)(1-3 mg/ml)，此外，转基因男性被激素诱导，并被证明产生0.85 mg/ml的LAtPA。乳蛋白凝胶显示乳制品(酪蛋白、免疫球蛋白)基本正常。这些实验表明，与正常妊娠和哺乳计划所需的时间相比，该载体在奶山羊身上的表达数据可以在更短的时间内得到评估，并可用于确定正常哺乳期间转基因在乳腺组织中的相对表达。
Three transgenic females from a first generation transgenic male were induced to lactate between Il and 12 months of age using a series of estrogen and progesterone injections. The milk contained human longer acting tissue plasminogen activator (LAtPA) at comparable concentrations (1-3 mg/ml) as occurred in the original founder female, In addition, the transgenic male was induced with a hormonal regime and was shown to produce 0.85 mg/ml of LAtPA. Milk protein gels indicated that the milk products (casein, IgG) were essentially normal. These experiments show that expression data for this vector can be evaluated in a shorter period of time in dairy goats than would be required through normal gestation and lactation schedules and can be used to identify the relative expression of transgenes in mammary tissue that would occur during normal lactation.