Proteome Analysis of Rat Bone Marrow Mesenchymal Stem Cell Differentiation
Proteome Analysis of Rat Bone Marrow Mesenchymal Stem Cell Differentiation
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DOI：
10.1021/pr100506u
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发表时间：
2010-10-01
影响因子：
4.4
通讯作者：
Elcin, Y. Murat
中科院分区：
文献类型：
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作者：
Celebi, Betul;Elcin, A. Eser;Elcin, Y. Murat
骨髓多能基质细胞（或间充质干细胞; MSC）具有更新的能力和在培养中分化成几种细胞类型的潜力，包括成骨细胞、软骨细胞、脂肪细胞、心肌细胞和神经元。本研究旨在观察大鼠骨髓间充质干细胞向成脂细胞分化过程中的蛋白表达谱，（通过地塞米松、异丁基甲基黄嘌呤、胰岛素和吲哚美辛），心肌原性（通过5-氮杂胞苷），软骨形成（通过抗坏血酸，胰岛素-转铁蛋白-亚硒酸和转化生长因子-β 1），和成骨（通过地塞米松、β-甘油磷酸盐和抗坏血酸）通过众所周知的分化诱导剂分化谱系。采用双向凝胶电泳（2-DE）分离分化后的MSCs蛋白质，并用Sypro Ruby染料检测蛋白质斑点。当相应点的表达（在第1和2周之间，第1和3周之间，第1和4周之间）显示增加（>= 2倍）或减少（>= 2倍）时，确定为上调或下调的蛋白质点（
Bone marrow multipotent stromal cells (or mesenchymal stem cells; MSCs) have the capacity for renewal and the potential to differentiate in culture into several cell types including osteoblasts, chondrocytes, adipocytes, cardiomyocytes, and neurons. This study was designed to investigate the protein expression profiles of rat bone marrow MSCs during differentiation into adipogenic (by dexamethasone, isobutylmethylxanthine, insulin, and indomethacin), cardiomyogenic (by 5-azacytidine), chondrogenic (by ascorbic acid, insulin-transferrin-selenous acid, and transforming growth factor-beta 1), and osteogenic (by dexamethasone, beta-glycerophosphate, and ascorbic acid) lineages by well-known differentiation inducers. Proteins extracted from differentiated MSCs were separated using two-dimensional gel electrophoresis (2-DE) and protein spots were detected using Sypro Ruby dye. Protein spots that were determined to be up- or down-regulated when the expression of corresponding spots (between weeks 1 and 2, 1 and 3, 1 and 4) showed an increase (>= 2-fold) or decrease (