Enterococcal cytolysin: activities and association with other virulence traits in a pathogenicity island.
Enterococcal cytolysin: activities and association with other virulence traits in a pathogenicity island.
复制标题
DOI：
10.1078/1438-4221-00301
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
N. Shankar;P. Coburn;C. Pillar;Wolfgang Haas;M. Gilmore
中科院分区：
文献类型：
--
作者：
N. Shankar;P. Coburn;C. Pillar;Wolfgang Haas;M. Gilmore
肠球菌溶细胞素是一种结构新颖的细菌毒素，由某些肠球菌表达。粪球菌，并且与称为羊毛硫抗生素的细菌素类有远亲关系。溶细胞素可以由大的信息素反应质粒编码，也可以位于致病岛内的染色体上。它是通过一个复杂的过程产生的，涉及8个基因的产物，命名为cylR 1，cylR 2，cylLL，cylLS，cylM，cylB，cylA和cylI。溶细胞素毒素、成熟和调节基因被组织成两种不同的转录物：结构转录物cylLLLSMBAI和调节转录物cylR 1 R2。活性溶细胞素亚基CylLL-和CylLS-以核糖体方式合成为不相同的肽，经后修饰，然后分泌和活化。溶细胞素操纵子被两种蛋白质CylR 1和CylR 2的活性抑制，并被涉及分泌的自诱导物CylLS的群体感应过程去抑制。E.粪肠球菌致病岛与其他毒力决定因子有关，包括聚集物质和肠球菌表面蛋白，ESP。
Enterococcal cytolysin is a structurally novel bacterial toxin expressed by some strains of E. faecalis and is distantly related to the class of bacteriocins known as lantibiotics. The cytolysin can be encoded by large pheromone-responsive plasmids, or on the chromosome within pathogenicity island. It is produced by a complex process that involves the products of eight genes, designated cylR1, cylR2, cylLL, cylLS, cylM, cylB, cylA, and cylI. The cytolysin toxin, maturation and regulatory genes are organized into two divergent transcripts: a structural transcript cylLLLSMBAI, and a regulatory transcript cylR1R2. The active cytolysin subunits, CylLL" and CylLS", are synthesized ribosomally as non-identical peptides, post-translationally modified, then secreted and activated. The cytolysin operon is repressed by the activities of two proteins, CylR1 and CylR2, and derepressed by a quorum-sensing process involving secreted autoinducer CylLS". The cytolysin operon within the E. faecalis pathogenicity island is associated with other virulence determinants, including aggregation substance and enterococcal surface protein, Esp.