A BAND OF F-ACTIN CONTAINING PODOSOMES IS INVOLVED IN BONE-RESORPTION BY OSTEOCLASTS
A BAND OF F-ACTIN CONTAINING PODOSOMES IS INVOLVED IN BONE-RESORPTION BY OSTEOCLASTS
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DOI：
10.1016/8756-3282(90)90082-a
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发表时间：
1990-01-01
期刊：
影响因子：
4.1
通讯作者：
TAKEUCHI, H
中科院分区：
文献类型：
--
作者：
KANEHISA, J;YAMANAKA, T;TAKEUCHI, H
离体兔破骨细胞培养灭活薄骨片挖掘吸收陷窝，可以可视化与明场或相差显微镜。叠加的明场图像的吸收陷窝和相应的破骨细胞的荧光图像固定和染色后，罗丹明共轭鬼笔环肽显示，一个明亮的荧光带的F-肌动蛋白的含有podosomes精确地概述了吸收陷窝在静止的破骨细胞。当吸收陷窝向外侧延伸时，清晰描绘的足粒带对应于吸收破骨细胞的前缘和最近的陷窝缺失部分。亮带的重塑和重组先于陷窝的侧边界的发展。形成这些带的足体是高度动态的，大小和位置不断变化，不断出现和消失。它们的寿命在2和12分钟之间变化。在体内生长的颅骨内表面上的骨吸收破骨细胞中也可以看到类似的条带。降钙素处理后，破骨细胞内的足状体消失，荧光带出现。我们的研究结果表明，podosomes是破骨细胞的吸收装置的重要组成部分。
Isolated rabbit osteoclasts cultured on devitalized thin bone slices excavate resorption lacunae that can be visualized with brightfield or phase-contrast microscopy. Superimposition of the brightfield images of such resorption lacunae and the flourescence images of the corresponding osteoclasts after fixation and staining with rhodamine-conjugated phalloidin revealed that a bright fluorescent band of F-actin-containing podosomes precisely outlined the resorption lacunae in stationary osteoclasts. When the resorption lacunae were being extended laterally, the clearly delineated band of podosomes corresponded to the advancing edge of the resorbing osteoclast and the most recently exacavated part of the lacunae. Reshaping and reorganization of the bright bands preceded development of the lateral boundary of the lacunae. Podosomes forming these bands were highly dynamic, changed in size and location, and appeared and disappeared continuously. Their lifespan varied between 2 and 12 min. Similar bands were also seen in vivo in bone-resorbing osteoclasts on the endocranial surface of growing calvariae. Podosomes disappeared in osteoclasts treated with calcitonin, resulting in the distribution of the fluorescent bands. Our results suggest that podosomes are an essential part of the resorption apparatus of osteoclasts.