Characterization of pericentrin isoforms in vivo
Characterization of pericentrin isoforms in vivo
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DOI：
10.1016/j.bbrc.2006.10.101
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发表时间：
2006-12-22
影响因子：
3.1
通讯作者：
Ogawa, Norio
中科院分区：
文献类型：
--
作者：
Miyoshi, Ko;Asanuma, Masato;Ogawa, Norio
周围素最初被鉴定为小鼠中心体蛋白，现在被称为周围素A。在人类中，具有C端钙调素结合域的更大的同源蛋白后来被鉴定为周中心素B。周围素被证明是纤毛发生的关键成分之一。但在体内，周丝蛋白的产品仍然含糊不清。在这里，我们描述了小鼠的胞外集落蛋白亚型。在小鼠组织Northern blots上识别出两个9.5kb和6.9kb的周中心素转录本，而周中心素A和B共享的5‘端序列的cRNA探针未能与6.9kb的信息杂交。鉴定出两个周丝蛋白基因，分别编码周丝蛋白B和一个新的异构体--周丝蛋白S，它与周丝蛋白B具有一个C端的钙调蛋白结合基序。免疫沉淀分析发现3个360、255和250 kDa的胞外蛋白分别对应于胞外蛋白B、胞外蛋白S和一个未知的N-末端产物。(C)2006 Elsevier Inc.保留所有权利。
Pericentrin was first identified as a mouse centrosomal protein and is now referred to as pericentrin A. A larger homologous protein in humans with a C-terminal calmodulin-binding domain was later identified as pericentrin B. Pericentrin has been shown to be one of the key components in ciliogenesis.. but in vivo pericentrin products have remained ambiguous. Here we characterized pericentrin isoforms in mice. Two pericentrin transcripts of 9.5 and 6.9 kb were recognized on the mouse tissue Northern blots, while a cRNA probe for a 5'-terminal sequence shared by pericentrin A and B failed to hybridize to the 6.9-kb message. Two pericentrin cDNAs were identified, which encoded pericentrin B and a novel isoform, pericentrin S, sharing with pericentrin B a C-terminal calmodulin-binding motif. Three pericentrin proteins of 360, 255, and 250 kDa revealed by immunoprecipitation analysis were thought to correspond to pericentrin B, pericentrin S, and an unknown N-terminal product. (c) 2006 Elsevier Inc. All rights reserved.