Antiproliferative activity of Pteleopsis suberosa leaf extract and its flavonoid components in human prostate carcinoma cells
Antiproliferative activity of Pteleopsis suberosa leaf extract and its flavonoid components in human prostate carcinoma cells
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DOI：
10.1055/s-2006-931556
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发表时间：
2006-06-01
期刊：
影响因子：
2.7
通讯作者：
Russo, Alessandra
中科院分区：
文献类型：
--
作者：
De Leo, Marinella;Braca, Alessandra;Russo, Alessandra
在本工作中，我们描述了鸡冠花叶提取物的化学成分及其对雄激素不敏感的人前列腺癌细胞(DU-145)的生物活性。植物叶的甲醇提取物具有抗肿瘤细胞生长的活性。这种活性提取物的分离得到了16个黄酮类化合物，包括没食子儿茶素和以山奈酚、栎素和杨梅素为苷元的黄酮醇。其中杨梅素3-O-(3‘-乙酰基)-α-L-阿拉伯吡喃糖苷(1)和杨梅素3-O-(4’-乙酰基)-α-L-阿拉伯吡喃糖苷(2)为首次报道。杨梅素3-O-α-L-鼠李糖苷(4)、杨梅素3-O-β-D-半乳糖苷(7)、杨梅素3-O-(6‘-没食子酰基)-β-D-半乳糖苷(9)、杨梅素3-O-α-D-木糖吡喃糖苷(10)、杨梅素3-O-α-L-阿拉伯呋喃糖苷(12)和半乳糖儿茶素(14)具有显著的抗肿瘤活性。
In this work we describe the chemical composition of Pteleopsis suberosa (Combretaceae) leaf extract and its biological activity against androgen-insensitive human prostate cancer cells (DU-145). The methanol extract of the plant leaves exhibited activity against tumor cell growth. Fractionation of this active extract led to the isolation and identification of sixteen flavonoids, including gallocatechin and flavonols having kaempferol, quercetin, and myricetin as aglycones. Among the myricetin derivatives, myricetin 3 -O-(3'-acetyl)-alpha-L-arabinopyranoside (1) and myricetin 3-O-(4'-acetyl)-alpha-L-arabinopyranoside (2) are now reported for the first time. Six compounds, myricetin 3-O-alpha-L-rhamnopyranoside (4), myricetin 3-O-beta-D-galactopyranoside (7), myricetin 3-O-(6'-galloyl)-beta-D-galactopyranoside (9), myricetin 3-O-beta-D-xylopyranoside (10), myricetin 3-O-alpha-L-arabinofuranoside (12), and gallocatechin (14), exhibited significant activity, reducing cell vitality and inducing apoptosis via the caspase-dependent pathway in DU-145 cells that can be, in part, correlated to modulation of redox-sensitive mechanisms.