Human transferrin: cDNA characterization and chromosomal localization.
Human transferrin: cDNA characterization and chromosomal localization.
复制标题
DOI：
10.1073/pnas.81.9.2752
复制
发表时间：
1984-05
影响因子：
11.1
通讯作者：
Funmei Yang;J. Lum;J. McGill;C. Moore;S. Naylor;P. H. V. Bragt;W. D. Baldwin;B. Bowman
中科院分区：
文献类型：
--
作者：
Funmei Yang;J. Lum;J. McGill;C. Moore;S. Naylor;P. H. V. Bragt;W. D. Baldwin;B. Bowman
转铁蛋白(Tf)是脊椎动物血清中主要的铁结合蛋白。它与其他四种蛋白质具有相同的氨基酸序列：乳转铁蛋白、卵转铁蛋白、黑色素瘤抗原p97和HuBlym-1。P97抗原和Tf受体基因已被定位在人类3号染色体上。本研究的目的是通过鉴定和鉴定Tf基因的cDNA并定位其染色体位置来启动对Tf基因的鉴定。用混合寡核苷酸探针筛选成人肝脏文库，获得了含有人Tf基因的重组表达载体。在分析的2.3kb的Tf cDNA中，有一个可能的前导序列，由57个核苷酸编码，后面是2037个核苷酸，编码同源的氨基和羧基结构域。在进化过程中，同源氨基和羧基结构域的三个区域被高度保守，可能反映了与铁结合相关的功能限制。染色体原位杂交定位和体细胞杂交分析表明，Tf基因位于人3号染色体上的q21-25，与Tf、Tf受体和黑色素瘤p97基因连锁一致。
Transferrin (Tf) is the major iron binding protein in vertebrate serum. It shares homologous amino acid sequences with four other proteins: lactotransferrin, ovotransferrin, melanoma antigen p97, and HuBlym-1. Antigen p97 and the Tf receptor genes have been mapped on human chromosome 3. The goal of the study described here was to initiate the characterization of the Tf gene by identifying and characterizing its cDNA and mapping its chromosomal location. Recombinant plasmids containing human cDNA encoding Tf have been isolated by screening an adult human liver library with a mixed oligonucleotide probe. Within the 2.3 kilobase pairs of Tf cDNA analyzed, there is a probable leader sequence encoded by 57 nucleotides followed by 2037 nucleotides that encode the homologous amino and carboxyl domains. During evolution, three areas of the homologous amino and carboxyl domains have been strongly conserved, possibly reflecting functional constraints associated with iron binding. Chromosomal mapping by in situ hybridization and somatic cell hybrid analysis indicate that the Tf gene is located at q21-25 on human chromosome 3, consistent with linkage of the Tf, Tf receptor, and melanoma p97 loci.