HIGH-PERFORMANCE ELECTROPHORESIS - ELIMINATION OF ELECTROENDOSMOSIS AND SOLUTE ADSORPTION
HIGH-PERFORMANCE ELECTROPHORESIS - ELIMINATION OF ELECTROENDOSMOSIS AND SOLUTE ADSORPTION
复制标题
DOI：
10.1016/s0021-9673(01)95485-8
复制
发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
HJERTEN, S
中科院分区：
文献类型：
--
作者：
HJERTEN, S
在理想的自由溶液电泳中，不应发生电内渗或溶质吸附到电泳室内部。在本文中，我们表明，当窄孔电泳管涂有单分子非交联聚丙烯酰胺层时，这两种干扰现象在自由高性能电泳中可以忽略不计。详细描述了涂层过程。
In ideal electrophoresis in free solution neither electroendosmosis nor adsorption of solutes onto the inside of the electrophoresis chamber should occur. In this paper we show that these two disturbing phenomena are negligible in free high-performance electrophoresis when the narrow-bore electrophoresis tube is coated with a mono-molecular layer of non-cross-linked polyacrylamide. The coating procedure is described in detail.