ESTIMATION OF NUCLEAR POPULATION FROM MICROTOME SECTIONS
ESTIMATION OF NUCLEAR POPULATION FROM MICROTOME SECTIONS
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DOI：
10.1002/ar.1090940210
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发表时间：
1946-01-01
影响因子：
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通讯作者：
ABERCROMBIE, M
中科院分区：
文献类型：
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作者：
ABERCROMBIE, M
只有在考虑细胞核大小的情况下，才有可能根据切片机切片中的细胞核计数来估计组织的细胞核群密度和准确比较不同组织中的细胞核数量。通常使用的从切片机切片中观察到的细胞核数量外推来估计细胞核群体密度的方法严重夸大了真实密度。核布居的真密度，即把每个核看作单个几何点时单位体积内的核数，必须求出。描述了两种方法来获得它。（1）平均核长度通过在与计数的切片成直角的切片中测量来获得，并应用于公式[图像]，其中P是每个切片的核点数，A是切片中核的表观数量的粗略计数，M是切片的厚度，L是核的平均长度。一个系统的，但在核的情况下，这是球体或定向椭球，小的误差。(2)计数2种不同厚度切片中的视核数。计数的差异是与切片厚度差异一样厚的切片中的核点的数量。
The estimation of density of nuclear population of tissues and the accurate comparison of the number of nuclei in different tissues, from counts of nuclei in microtome sections, are only possible if size of nucleus is taken into account. The commonly used method of estimating the density of nuclear population by extrapolation from the numbers of nuclei seen in a microtome section grossly exaggerates the true density. The true density of nuclear population, that is the number of nuclei per unit volume when each nucleus is taken as a single geometrical point, must be obtained. Two methods are described for getting it. (1) The mean nuclear length is obtained by measurement in sections cut at right angles to those counted, and applied in the formula [image] where P is the number of nuclear points per section, A the crude count of apparent number of nuclei in the section, M the thickness of section and L the average length of the nuclei. A systematic but, in the case of nuclei which are spheres or orientated ellipsoids, small error is involved. (2) The apparent number of nuclei in sections of 2 different thickness are counted. The difference in the counts is the number of nuclear points in a section as thick as the difference in thickness of the sections.