MicroRNA1917-CTR1-LIKE PROTEIN KINASE 4 impacts fruit development via tuning ethylene synthesis and response
MicroRNA1917-CTR1-LIKE PROTEIN KINASE 4 impacts fruit development via tuning ethylene synthesis and response
复制标题
MicroRNA1917-CTR1-LIKE 蛋白激酶 4 通过调节乙烯合成和反应影响果实发育
DOI：
10.1016/j.plantsci.2019.110334
复制
发表时间：
2019
期刊：
影响因子：
5.2
通讯作者：
Zhanhui Zhang
中科院分区：
文献类型：
--
作者：
Tianxiao Yang;Yongyan Wang;Haiping Liu;Wen Zhang;Mao Chai;Guiliang Tang;Zhanhui Zhang
MicroRNA1917(MiR1917)是新发现的调节番茄乙烯反应的miRNAs。然而，关于其在果实发育和成熟中的作用，证据仍然有限。在这里，我们研究了miR1917-slCTR4模块在番茄果实发育中的可能作用。我们通过表达短串联靶模拟(STTM1917)产生了miR1917击倒突变体。QRT-PCR和Northern-Blot分析表明，miR1917在STTM1917中表达下调。同时，miR1917靶向的SLCTR4基因表达上调。STTM1917植株表现出一系列的发育表型，包括更大的生物量，更长的顶生小叶，更大的花器官，增大的果实和种子大小。RNA-seq和qRT-PCR分析表明，与野生型相比，转基因株系中大量miRNAs和基因的表达水平发生了显著变化。这些miRNAs和基因包括与果实发育相关的miRNAs、与果实成熟相关的转录因子和乙烯代谢基因。综上所述，我们的结果表明，miR1917与成熟调节剂协同工作，可能调控乙烯途径中的多个基因，从而调控果实发育。我们的结果进一步表明，通过STTM微调miRNAs的表达可以应用于番茄的农艺改良。
MicroRNA1917 (miR1917) is a newly identified miRNAs that regulate ethylene responses in tomato. However, evidence is still limited about its functions in fruit development and ripening. Here, we investigated the possible roles of miR1917-SlCTR4module in tomato fruit development. We generated miR1917 knock-down mutants by expressing Short Tandem Target Mimic (STTM1917). qRT-PCR and northern-blot analyses suggested that the expression of miR1917 are down-regulated in STTM1917. Concomitantly, miR1917-targetedSlCTR4gene was up-regulated. STTM1917 plants showed a series of developmental phenotypes, including larger biomass, longer terminal leaflet, bigger floral organ and enhanced fruit and seed size. RNA-seq and qRT-PCR analyses suggested that the expression levels of numerous miRNAs and genes in the transgenic line were significantly altered compared to the wild type. These miRNAs and genes include fruit development-related miRNAs, fruit ripening-related transcription factors and ethylene metabolism genes. Altogether, our results demonstrated that working in concert with ripening regulators, miR1917 might regulate multiple genes in ethylene pathway, thereby modulating fruit development. Our results further indicated that fine-tuning miRNAs expression via STTM can be deployed for agronomic improvement of tomato.