Earthworm compound and rock biofertilizer enriched in nitrogen by inoculation with free living diazotrophic bacteria
Earthworm compound and rock biofertilizer enriched in nitrogen by inoculation with free living diazotrophic bacteria
复制标题
通过接种自由活固氮菌而富氮的蚯蚓复合物和岩石生物肥料
DOI：
10.1007/s11274-010-0357-z
复制
发表时间：
2010
影响因子：
4.1
通讯作者：
P. Straaten
中科院分区：
文献类型：
--
作者：
F. S. Lima;N. Stamford;C. Sousa;M. A. Lira Junior;S. Malheiros;P. Straaten
自由生活的重氮营养细菌可以富集有机质源中的氮。本文报道了岩石生物肥料与两种有机物（蚯蚓化合物和冰淇淋废料）混合接种游离重氮营养菌的试验。蚯蚓复合肥料与磷、钾生物肥料混合，形成基质S1（蚯蚓复合肥料3 dm3+ PK生物肥料1 dm3，废冰淇淋1 dm3）；S2（蚯蚓化合物2.5 dm3+ PK生物肥料1.5 dm3和废冰淇淋1 dm3L）和s3蚯蚓化合物2.0 dm3+ PK生物肥料2.0 dm3和废冰淇淋1 dm3)，随后用从巴西不同土壤中分离的3种自由活重氮营养细菌接种（100 mL锅−1）。对照组是一种未接种的蚯蚓化合物。在不同的培养时间（0、15、30和45天）收集样品，分析总氮含量。总氮浓度在S1、s2和s3底物中分别在34、27和29天最高。菌株nfb1001对所有基质的总氮均有提高作用，其中以蚯蚓化合物含量最高的s1基质在34 d时效果最好。菌株生长30 d后氮含量下降，为生产有机肥的最佳时期。利用游离重氮营养菌富氮的有机生物肥料具有重要的有机农业应用价值。
Free living diazotrophic bacteria are known to enrich nitrogen of organic matter sources. In this paper we report of experiments using rock biofertilizers mixed with two types of organic matter (earthworm compound and ice cream waste) inoculated with free living diazotrophic bacteria. The earthworm compound and P and K biofertilizers were mixed to form substrates S1(earthworm compound 3 dm3+ PK biofertilizer 1 dm3and waste ice cream 1 dm3); S2(earthworm compound 2.5 dm3+ PK biofertilizer 1.5 dm3and waste ice cream 1 dm3L) and S3earthworm compound 2.0 dm3+ PK biofertilizer 2.0 dm3and waste ice cream 1 dm3), and subsequently inoculated (100 mL pot−1) with 3 free living diazotrophic bacteria isolated from different Brazilian soils. The control was an uninoculated earthworm compound. Samples were collected at various incubation time (0; 15; 30 and 45 days) and analyzed for total N. Total N concentrations were highest in S1, S2and S3substrates at 34, 27 and 29 days, respectively. The isolate NFB 1001 increased total N in all substrates and the best results were obtained at 34 days in S1substrate which contained the highest amount of earthworm compound. The isolates promoted a decline in N content after 30 days of growth, indicating the best time to produce the organic biofertilizer. The organic biofertilizer enriched in nitrogen by free living diazotrophic bacteria is of relevance to organic agriculture.