A whole blood immunoassay using gold nanoshells
A whole blood immunoassay using gold nanoshells
复制标题
DOI：
10.1021/ac0262210
复制
发表时间：
2003-05-15
影响因子：
7.4
通讯作者：
West, J
中科院分区：
文献类型：
--
作者：
Hirsch, LR;Jackson, JB;West, J
一种能够在复杂生物介质中检测分析物而无需任何样品制备的快速免疫分析方法是DES m-Bed。这是通过使用金纳米壳来实现的，这是一种分层的介电金属纳米颗粒，其光学共振是其组成层的相对大小的函数。在分析物存在的情况下，用光谱监测了具有近红外消光光谱的抗体/纳米壳结合物的聚集。在生理盐水、血清和全血中成功检测到免疫球蛋白。该系统构成了一种简单的免疫分析方法，能够在10-30分钟内检测到不同介质中每毫升亚纳克数量的各种分析物。
A rapid immunoassay capable of detecting analyte within complex biological media without any sample preparation is des m-bed. This was accomplished using gold nanoshells, layered dielectric-metal nanoparticles whose optical resonance is a function of the relative size of its constituent layers. Aggregation of antibody/nanoshell conjugates with extinction spectra in the near-infrared was monitored spectroscopically in the presence of analyte. Successful detection of immunoglobulins was achieved in saline, serum, and whole blood. This system constitutes a simple immunoassay capable of detecting sub-nanogram-per-milliliter quantities of various analytes in different media within 10-30 min.