Selective expression of Knot/Collier, a transcriptional regulator of the EBF/Olf-1 family, endows the Drosophila sensory system with neuronal class-specific elaborated dendritic patterns
Selective expression of Knot/Collier, a transcriptional regulator of the EBF/Olf-1 family, endows the Drosophila sensory system with neuronal class-specific elaborated dendritic patterns
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DOI：
10.1111/j.1365-2443.2007.01107.x
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发表时间：
2007-09-01
期刊：
影响因子：
2.1
通讯作者：
Uemura, Tadashi
中科院分区：
文献类型：
--
作者：
Hattori, Yukako;Sugimura, Kaoru;Uemura, Tadashi
树突树形态是神经系统细胞多样性的标志，果蝇树突树突（da）神经元为研究其分子基础提供了一个很好的模型系统。数据神经元按照分支复杂度的递增顺序分为I-IV类。作为早期b细胞因子(EBF)/嗅觉1 （Olf-1）家族的转录调节因子，Knot (Kn)/Collier （Col）在IV类神经元中选择性表达，IV类神经元产生四类神经元中最广泛和最复杂的树突树。IV类神经元失去kn功能后，其树突分支数量大大减少。相反，在I类和II类中，错表达kn会产生额外的高阶分支，而在iii类中，错表达kn几乎不会形成特定的短分支和直分支。kn的功能丧失和错误表达都与另外两种转录调节因子突变（Ab）和切割（Ct）的表达模式的显著改变无关，这两种转录调节因子在树突树的形成中起着重要作用，具有与kn不同的类特异性。相反，Kn对于驱动编码iv类特异性通道蛋白的基因的表达是必要和充分的。总的来说，我们所有的结果都表明，Kn发挥其细胞自主功能来控制iv类精细树突乔木的形成，并可能控制其功能。
Dendritic tree morphology is a hallmark of cellular diversity in the nervous system, and Drosophila dendritic arborization (da) neurons provide an excellent model system to study its molecular basis. The da neurons are classified into four classes I-IV in the order of increasing branching complexity. A transcriptional regulator of the early B-cell factor (EBF)/olfactory 1 (Olf-1) family, Knot (Kn)/Collier (Col) is expressed selectively in class IV neurons, which generate the most expansive and complicated dendritic trees in the four classes. Loss of kn function in class IV neurons greatly reduced the number of their dendritic branches. Conversely mis-expression of kn in classes I and II produced supernumerary higher-order branches, whereas class III-specific short and straight terminal branches was hardly formed by kn mis-expression. Neither kn loss of function nor mis-expression were associated with dramatic alterations in the expression patterns of two other transcriptional regulators, Abrupt (Ab) and Cut (Ct), which play important roles in shaping dendritic trees with distinct class specificity from Kn. In contrast, Kn was necessary and sufficient to drive expression of a gene that encodes a class IV-specific channel protein. Collectively, all of our results suggest that Kn exerts its cell-autonomous function to control the formation, and possibly the function, of class IV-like elaborated dendritic arbors.