Root and butt rot of Todo fir (Abies sachalinensis) caused by Heterobasidion annosum s.l. in Hokkaido, Japan
Root and butt rot of Todo fir (Abies sachalinensis) caused by Heterobasidion annosum s.l. in Hokkaido, Japan
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DOI：
10.1111/j.1439-0329.2007.00492.x
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发表时间：
2007-06
期刊：
影响因子：
1.4
通讯作者：
S. Tokuda;Y. Ota;T. Hattori
中科院分区：
文献类型：
--
作者：
S. Tokuda;Y. Ota;T. Hattori
摘要对日本北海道68年生东道杉木人工林35 × 30 m地块的根腐病和枯腐病的发生及症状进行了调查。47%的砍下的树桩发生了腐烂，52%的树桩表现出类似的腐烂特征，其颜色为黄橙色至浅棕色，心材中有膨胀的口袋。从腐烂中分离的纯培养物的形态特征与在研究地点外的倒落原木上发现的杂交藤（Heterobasidion annosum sensu lato）担子果脯分离的培养物相似。另外，对3个基因位点（甘油醛3-磷酸脱氢酶、热休克蛋白80-1和延伸因子1- α基因）的DNA分析表明，这3个基因位点与日本血吸虫的分离株属于同一进化支。它们形成了小孢子虫的一个亚支（欧洲S群的小孢子虫）。本文首次报道了从日本某人工林根腐和枯腐中分离得到的羊草的分子鉴定。
Summary The occurrence and symptoms of root and butt rot were examined in a 35 × 30 m plot of 68-year-old Todo fir plantation in Hokkaido, Japan. Forty-seven percent of the cut stumps were decayed and 52% of the decayed stumps showed similar decay characteristics with yellowish orange to light brown colouration and expanded pockets in the heartwood. Morphological characteristics of the pure cultures isolated from the decay were similar to the cultures isolated from basidiocarps of Heterobasidion annosum sensu lato, found on fallen logs outside of the research site. Also DNA analysis based on the combined data set of three gene loci (glyceraldehyde 3-phosphate dehydrogenase, heat shock protein 80–1 and elongation factor 1-alpha genes) showed that the isolates from the decay are included in the same clade with the Japanese H. annosum s.l. isolates. They form a subclade to H. parviporum (the European S group of H. annosum s.l.). This is the first report of molecular determination of H. annosum s.l. isolated from root and butt rot in a plantation in Japan.